A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli

Kari L Schmidt; Nicholas D Peterson; Ryan J Kustusch; Mark C Wissel; Becky Graham; Gregory J Phillips; David S Weiss

doi:10.1128/jb.186.3.785-793.2004

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A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli

Journal article

Open access

Peer reviewed

A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli

Kari L Schmidt, Nicholas D Peterson, Ryan J Kustusch, Mark C Wissel, Becky Graham, Gregory J Phillips and David S Weiss

Journal of bacteriology, Vol.186(3), pp.785-793

02/2004

DOI: 10.1128/jb.186.3.785-793.2004

PMCID: PMC321481

PMID: 14729705

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Abstract

FtsE and FtsX have homology to the ABC transporter superfamily of proteins and appear to be widely conserved among bacteria. Early work implicated FtsEX in cell division in Escherichia coli, but this was subsequently challenged, in part because the division defects in ftsEX mutants are often salt remedial. Strain RG60 has an ftsE::kan null mutation that is polar onto ftsX. RG60 is mildly filamentous when grown in standard Luria-Bertani medium (LB), which contains 1% NaCl, but upon shift to LB with no NaCl growth and division stop. We found that FtsN localizes to potential division sites, albeit poorly, in RG60 grown in LB with 1% NaCl. We also found that in wild-type E. coli both FtsE and FtsX localize to the division site. Localization of FtsX was studied in detail and appeared to require FtsZ, FtsA, and ZipA, but not the downstream division proteins FtsK, FtsQ, FtsL, and FtsI. Consistent with this, in media lacking salt, FtsA and ZipA localized independently of FtsEX, but the downstream proteins did not. Finally, in the absence of salt, cells depleted of FtsEX stopped dividing before any change in growth rate (mass increase) was apparent. We conclude that FtsEX participates directly in the process of cell division and is important for assembly or stability of the septal ring, especially in salt-free media.

Cell Cycle Proteins - analysis

Cell Division

Cystic Fibrosis Transmembrane Conductance Regulator - physiology

Escherichia coli Proteins - physiology

ATP-Binding Cassette Transporters - physiology

Cell Cycle Proteins - physiology

Escherichia coli - physiology

Escherichia coli Proteins - analysis

Cystic Fibrosis Transmembrane Conductance Regulator - analysis

ATP-Binding Cassette Transporters - analysis

Bacterial Proteins

Protein Transport

Details

Title: Subtitle: A predicted ABC transporter, FtsEX, is needed for cell division in Escherichia coli
Creators: Kari L Schmidt - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
Nicholas D Peterson
Ryan J Kustusch
Mark C Wissel
Becky Graham
Gregory J Phillips
David S Weiss
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.186(3), pp.785-793
DOI: 10.1128/jb.186.3.785-793.2004
PMID: 14729705
PMCID: PMC321481
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Publisher: United States
Grant note: GM50836 / NIGMS NIH HHS R01 GM059893 / NIGMS NIH HHS GM59893 / NIGMS NIH HHS
Language: English
Date published: 02/2004
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001109202771

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