Journal article
A predominance of the T cell receptor V gamma 2/V delta 2 subset in human mycobacteria-responsive T cells suggests germline gene encoded recognition
The Journal of immunology (1950), Vol.147(10), pp.3360-3369
11/15/1991
DOI: 10.4049/jimmunol.147.10.3360
PMID: 1658147
Abstract
Little is known about the nature of Ag recognition by the TCR-gamma delta. The recent observation that gamma delta T cells preferentially recognize mycobacterial Ag provides a model to examine the molecular basis of gamma delta-TCR recognition. Here, examination of the Mycobacteria-stimulated peripheral blood T cells with TCR-specific mAb revealed a predominance of T cells bearing V gamma 2/V delta 2 gene products. PCR cloning and sequence analysis of the TCR chains demonstrated extensive junctional diversity indicating that the response was polyclonal. The marked in vitro gamma delta T cell response to Mycobacteria was also detected in newborns before encounters with foreign Ag and exclusively involved the same V-gene usage observed in adults. Together, these results suggest that a major mechanism of gamma delta T cell reactivity involves recognition mediated by germline-encoded segments of the TCR.
Details
- Title: Subtitle
- A predominance of the T cell receptor V gamma 2/V delta 2 subset in human mycobacteria-responsive T cells suggests germline gene encoded recognition
- Creators
- G Panchamoorthy - Laboratory of Immunochemistry, Dana-Farber Cancer Institute, Boston, MA 02115J McLeanR L ModlinC T MoritaS IshikawaM B BrennerH Band
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.147(10), pp.3360-3369
- DOI
- 10.4049/jimmunol.147.10.3360
- PMID
- 1658147
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 11/15/1991
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094482202771
Metrics
31 Record Views