Journal article
A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate
International journal of developmental neuroscience, Vol.65(1), pp.61-65
04/2018
DOI: 10.1016/j.ijdevneu.2017.10.001
PMID: 29031644
Abstract
•GDNF levels in human umbilical cord blood decrease with increasing gestational age.•CSF GDNF levels trend higher in preterm neonates with PVH as compared to those preterm neonates with mild or no brain injury on head ultrasound scan in this preliminary study.•A trend of higher GDNF levels was observed in term newborns when compared to preterm newborns at term or post term corrected gestational ages.
This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns’ umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions.
Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background.
Cord blood GDNF levels were significantly higher (p=0.004) in preterm newborns (n=6) (115.05±57.17,pg/ml) when compared to term newborns (n=14) (19.67±10.67,pg/ml). GDNF levels in CSF trended (p=0.07) higher in term newborns (n=10) (19.56±9.11,pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n=5) (14.49±3.53,pg/ml).
GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions.
Details
- Title: Subtitle
- A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate
- Creators
- Rahul Rajkumar - Wayne State UniversityBhavana Bhaya - Wayne State UniversityDivya Mamilla - Wayne State UniversityTheresa Czech - Wayne State UniversityEsther Kisseih - Wayne State UniversityArun Saini - Wayne State UniversityNitin Chouthai - Wayne State University
- Resource Type
- Journal article
- Publication Details
- International journal of developmental neuroscience, Vol.65(1), pp.61-65
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.ijdevneu.2017.10.001
- PMID
- 29031644
- ISSN
- 0736-5748
- eISSN
- 1873-474X
- Language
- English
- Date published
- 04/2018
- Academic Unit
- Stead Family Department of Pediatrics; Neurology (Pediatrics)
- Record Identifier
- 9984695677202771
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