Journal article
A recombinant Leishmania chagasi antigen that stimulates cellular immune responses in infected mice
Infection and immunity, Vol.63(5), pp.2062-2069
05/1995
DOI: 10.1128/IAI.63.5.2062-2069.1995
PMCID: PMC173265
PMID: 7729921
Abstract
Cellular immune mechanisms resulting in gamma interferon production are critical for protection against visceral leishmaniasis. Antigens stimulating T-cell responses are likely present in the intracellular amastigote form of the parasite, since this is the form found in a mammalian host. To identify T-cell antigens of Leishmania chagasi, the parasite causing South American visceral leishmaniasis, we used a double antibody-T-cell technique to screen an amastigote cDNA library. One cDNA selected (Lcr1) encodes an antigen that stimulated proliferation of splenic T lymphocytes from infected mice that were either resistant (C3H.HeJ) or susceptible (BALB/c) to L. chagasi infection. The Lcr1 cDNA contains four highly divergent 201-bp repeats homologous to the 204-bp repeat of a Trypanosoma cruzi flagellar antigen gene. Results are consistent with a single copy of the Lcr1 gene producing an mRNA of > 10 kb and a protein of > 200 kDa. Recombinant Lcr1, cloned adjacent to polyhistidine and purified on a nickel affinity column, stimulated gamma interferon but not interleukin-4 (IL-4), IL-5, or IL-10 secretion by T-cell-enriched splenocytes from either susceptible or resistant mice during L. chagasi infection. Immunization with Lcr1 partially protected BALB/c mice against challenge with L. chagasi, indicating the utility of the double screening approach in selecting relevant T-cell antigens.
Details
- Title: Subtitle
- A recombinant Leishmania chagasi antigen that stimulates cellular immune responses in infected mice
- Creators
- Mary E Wilson - Department of Internal Medicine, University of Iowa, Iowa City 52242, USABetty M Young - Department of Internal Medicine, University of Iowa, Iowa City 52242, USAKatherine Paetz Andersen - Department of Internal Medicine, University of Iowa, Iowa City 52242, USAJoel V Weinstock - Department of Internal Medicine, University of Iowa, Iowa City 52242, USAAhmed Metwali - Department of Internal Medicine, University of Iowa, Iowa City 52242, USAKamran M Ali - Department of Internal Medicine, University of Iowa, Iowa City 52242, USAJohn E Donelson - Department of Internal Medicine, University of Iowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.63(5), pp.2062-2069
- DOI
- 10.1128/IAI.63.5.2062-2069.1995
- PMID
- 7729921
- PMCID
- PMC173265
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 05/1995
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984002374502771
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