Journal article
A recombinant adenovirus expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis elicits strong antigen-specific immune responses in mice
Molecular immunology, Vol.62(1), pp.86-95
11/2014
DOI: 10.1016/j.molimm.2014.06.007
PMID: 24980867
Abstract
•Developed a novel adenoviral vector expressing Mtb antigens in native forms.•The adenoviral vector, Ad5-CEAB could elicit strong Th1 immune response.•Ad5-CEAB could enhance antigen-specific cellular, mucosal and humoral immunity in mice.•Ad5-CEAB holds promise as a TB vaccine candidate for further investigation.
Tuberculosis (TB) is caused by an infection of Mycobacterium tuberculosis (Mtb) and remains an enormous and increasing health burden worldwide. To date, Mycobacterium bovis Bacillus Calmette Guerin (BCG) is the only licensed anti-TB vaccine worldwide, which provides an important but limited protection from the Mtb infection. The development of alternative anti-TB vaccines is therefore urgently needed. Here we report, the generation of Ad5-CEAB, a recombinant adenovirus expressing Mtb antigens of CFP10, ESAT6, Ag85A and Ag85B proteins in a form of mixture. In order to evaluate the immunogenicity of Ad5-CEAB, mice were immunized with Ad5-CEAB by intranasal instillation three times with 2-week intervals. The results demonstrated that Ad5-CEAB elicited a strong antigen-specific immune response, particularly of the Th1 immune responses that were characterized by an increased ratio of IgG2a/IgG1 and secretions of Th1 type cytokines, IFN-γ, TNF-α, IL-2 and IL-12. In addition, the Ad5-CEAB also showed an ability to enhance humoral responses with a dramatically augmented antigen-specific serum IgG. Furthermore, an elevated sIgA were also found in the bronchoalveolar lavage fluid of the immunized mice, suggesting the elicitation of mucosal immune responses. These data indicate that Ad5-CEAB can induce a broad range of antigen-specific immune responses in vivo, which provides a promising and novel route for developing anti-TB vaccines and warrants further investigation.
Details
- Title: Subtitle
- A recombinant adenovirus expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis elicits strong antigen-specific immune responses in mice
- Creators
- Wu Li - Shanghai Jiao Tong UniversityGuangcun Deng - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, Ningxia 750021, ChinaMin Li - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, Ningxia 750021, ChinaJin Zeng - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, Ningxia 750021, ChinaLiping Zhao - Shanghai Jiao Tong UniversityXiaoming Liu - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, Ningxia 750021, ChinaYujiong Wang - Shanghai Jiao Tong University
- Resource Type
- Journal article
- Publication Details
- Molecular immunology, Vol.62(1), pp.86-95
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.molimm.2014.06.007
- PMID
- 24980867
- ISSN
- 0161-5890
- eISSN
- 1872-9142
- Grant note
- DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 31160515; name: National Key Basic Research Program of China, award: 2012CB126301, 2012CB518801; name: Key Technologies Research and Development Program of China, award: 2012BAD12B07-4
- Language
- English
- Date published
- 11/2014
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288792702771
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