A role for planar cell polarity signaling in angiogenesis

Pasquale Cirone; Shengda Lin; Hilary L Griesbach; Yi Zhang; Diane C Slusarski; Craig M Crews

doi:10.1007/s10456-008-9116-2

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A role for planar cell polarity signaling in angiogenesis

Journal article

Peer reviewed

A role for planar cell polarity signaling in angiogenesis

Pasquale Cirone, Shengda Lin, Hilary L Griesbach, Yi Zhang, Diane C Slusarski and Craig M Crews

Angiogenesis (London), Vol.11(4), pp.347-360

2008

DOI: 10.1007/s10456-008-9116-2

PMCID: PMC3547076

PMID: 18798004

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Abstract

The planar cell polarity (PCP) pathway is a highly conserved signaling cascade that coordinates both epithelial and axonal morphogenic movements during development. Angiogenesis also involves the growth and migration of polarized cells, although the mechanisms underlying their intercellular communication are poorly understood. Here, using cell culture assays, we demonstrate that inhibition of PCP signaling disrupts endothelial cell growth, polarity, and migration, all of which can be rescued through downstream activation of this pathway by expression of either Daam-1, Diversin or Inversin. Silencing of either Dvl2 or Prickle suppressed endothelial cell proliferation. Moreover, loss of p53 rescues endothelial cell growth arrest but not the migration inhibition caused by PCP disruption. In addition, we show that the zebrafish Wnt5 mutant (pipetail (ppt)), which has impaired PCP signaling, displays vascular developmental defects. These findings reveal a potential role for PCP signaling in the coordinated assembly of endothelial cells into vascular structures and have important implications for vascular remodeling in development and disease.

Neovascularization, Physiologic - drug effects

Blood Vessels - abnormalities

Cyclohexanes - pharmacology

Humans

Wnt Proteins - metabolism

Models, Animal

Cell Polarity - drug effects

Dishevelled Proteins

Cell Line

Tumor Suppressor Protein p53 - metabolism

Zebrafish

Phosphoproteins - genetics

Mutation - genetics

beta Catenin - metabolism

Caveolin 1 - metabolism

Cell Movement - drug effects

Animals

Signal Transduction - drug effects

Endothelial Cells - cytology

Adaptor Proteins, Signal Transducing - genetics

Blood Vessels - drug effects

Cell Proliferation - drug effects

Mice

Sesquiterpenes - pharmacology

Endothelial Cells - drug effects

Details

Title: Subtitle: A role for planar cell polarity signaling in angiogenesis
Creators: Pasquale Cirone - Department of Molecular, Cellular and Developmental Biology, Yale University, Kline Biology Tower, P.O. Box 208103, New Haven, CT 06520-8103, USA
Shengda Lin - University of Iowa
Hilary L Griesbach
Yi Zhang - Yale University
Diane C Slusarski
Craig M Crews
Resource Type: Journal article
Publication Details: Angiogenesis (London), Vol.11(4), pp.347-360
DOI: 10.1007/s10456-008-9116-2
PMID: 18798004
PMCID: PMC3547076
NLM abbreviation: Angiogenesis
ISSN: 0969-6970
eISSN: 1573-7209
Publisher: Germany
Grant note: R01 CA083049 / NCI NIH HHS CA112369 / NCI NIH HHS R01 CA112369 / NCI NIH HHS CA083049 / NCI NIH HHS
Language: English
Date published: 2008
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983991960102771

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