Journal article
A role for surfactant protein D in innate immunity of the human prostate
The Prostate, Vol.65(3), pp.241-251
2005
DOI: 10.1002/pros.20292
PMID: 15948134
Abstract
Objectives: Surfactant protein D (SP-D) is a member of the collectin family of proteins, which are involved in host defense mechanisms in the lung. In the present study, we found that SP-D is produced in the human prostate where it may play a role in innate immunity.
Methods and results: Using reverse-transcriptase PCR and Western blot analysis, we demonstrate that SP-D mRNA and protein are present in human prostate tissue. In situ hybridization and immunohistochemistry revealed that SP-D mRNA and protein are localized in epithelial cells of prostate glands. Prostate glands that are surrounded by inflammatory cells produce increased amounts of SP-D protein. We also show that SP-D inhibits the infection of LNCaP and P69SV40T prostate epithelial cells by Chlamydia trachomatis in an in vitro infection assay. Furthermore, using truncated human SP-D mutants, we demonstrate that SP-D binds to Chlamydia trachomatis via its carboxy-terminal lectin domains.
Conclusions: Our in vitro studies suggest that SP-D protects the prostate from infection by pathogens. SP-D protein levels are increased at sites of inflammation in the prostate, suggesting SP-D may also contribute more generally to inflammatory regulation in the prostate.
Details
- Title: Subtitle
- A role for surfactant protein D in innate immunity of the human prostate
- Creators
- Rebecca E OBERLEY - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, United StatesKelli L GOSS - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, United StatesLaila DAHMOUSH - Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa, United StatesKevin A AULT - Department of Obstetrics and Gynecology, University of Iowa College of Medicine, Iowa City, Iowa, United StatesErika C CROUCH - Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, United StatesJeanne M SNYDER - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, Iowa, United States
- Resource Type
- Journal article
- Publication Details
- The Prostate, Vol.65(3), pp.241-251
- Publisher
- Wiley-Liss; New York, NY
- DOI
- 10.1002/pros.20292
- PMID
- 15948134
- ISSN
- 0270-4137
- eISSN
- 1097-0045
- Language
- English
- Date published
- 2005
- Academic Unit
- Anatomy and Cell Biology; Stead Family Department of Pediatrics; Pathology; Urology
- Record Identifier
- 9984047673902771
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