Journal article
A secreted sirtuin from Campylobacter jejuni contributes to neutrophil activation and intestinal inflammation during infection
Science advances, Vol.9(32), pp.eade2693-eade2693
08/11/2023
DOI: 10.1126/sciadv.ade2693
PMCID: PMC10421069
PMID: 37566649
Abstract
Histone modifications control numerous processes in eukaryotes, including inflammation. Some bacterial pathogens alter the activity or expression of host-derived factors, including sirtuins, to modify histones and induce responses that promote infection. In this study, we identified a deacetylase encoded by Campylobacter jejuni which has sirtuin activities and contributes to activation of human neutrophils by the pathogen. This sirtuin is secreted from the bacterium into neutrophils, where it associates with and deacetylates host histones to promote neutrophil activation and extracellular trap production. Using the murine model of campylobacteriosis, we found that a mutant of this bacterial sirtuin efficiently colonized the gastrointestinal tract but was unable to induce cytokine production, gastrointestinal inflammation, and tissue pathology. In conclusion, these results suggest that secreted bacterial sirtuins represent a previously unreported class of bacterial effector and that bacterial-mediated modification of host histones is responsible for the inflammation and pathology that occurs during campylobacteriosis.
Details
- Title: Subtitle
- A secreted sirtuin from Campylobacter jejuni contributes to neutrophil activation and intestinal inflammation during infection
- Creators
- Sean M. Callahan - University of Tennessee at KnoxvilleTrevor J. Hancock - University of Tennessee at KnoxvilleRyan S. Doster - Vanderbilt University Medical CenterCaroline B. Parker - University of Tennessee at KnoxvilleMary E. Wakim - University of Tennessee at KnoxvilleJennifer A. Gaddy - Vanderbilt University Medical CenterJeremiah G. Johnson - University of Tennessee at Knoxville
- Resource Type
- Journal article
- Publication Details
- Science advances, Vol.9(32), pp.eade2693-eade2693
- Publisher
- Amer Assoc Advancement Science
- DOI
- 10.1126/sciadv.ade2693
- PMID
- 37566649
- PMCID
- PMC10421069
- ISSN
- 2375-2548
- eISSN
- 2375-2548
- Number of pages
- 22
- Grant note
- R01HD090061 / VA~Merit Award; US Department of Veterans Affairs 2019-67017-29261 / USDA NIFA; United States Department of Agriculture (USDA) UTK Start-Up Funds R01AI166535; K08AAI151100; I01 BX005352-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 08/11/2023
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696706402771
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