Journal article
A self-encoded capsid derivative restricts Ty1 retrotransposition in Saccharomyces
Current genetics, Vol.62(2), pp.321-329
05/01/2016
DOI: 10.1007/s00294-015-0550-6
PMCID: PMC4826814
PMID: 26650614
Abstract
Retrotransposons and retroviral insertions have molded the genomes of many eukaryotes. Since retroelements transpose via an RNA intermediate, the additive nature of the replication cycle can result in massive increases in copy number if left unchecked. Host organisms have countered with several defense systems, including domestication of retroelement genes that now act as restriction factors to minimize propagation. We discovered a novel truncated form of the Saccharomyces Ty1 retrotransposon capsid protein, dubbed p22 that inhibits virus-like particle (VLP) assembly and function. The p22 restriction factor expands the repertoire of defense proteins targeting the capsid and highlights a novel host-parasite strategy. Instead of inhibiting all transposition by domesticating the restriction gene as a distinct locus, Ty1 and budding yeast may have coevolved a relationship that allows high levels of transposition when Ty1 copy numbers are low and progressively less transposition as copy numbers rise. Here, we offer a perspective on p22 restriction, including its mode of expression, effect on VLP functions, interactions with its target, properties as a nucleic acid chaperone, similarities to other restriction factors, and future directions.
Details
- Title: Subtitle
- A self-encoded capsid derivative restricts Ty1 retrotransposition in Saccharomyces
- Creators
- David J. Garfinkel - University of GeorgiaJessica M. Tucker - University of GeorgiaAgniva Saha - University of GeorgiaYuri Nishida - University of GeorgiaKatarzyna Pachulska-Wieczorek - Institute of Bioorganic Chemistry, Polish Academy of SciencesLeszek Blaszczyk - Poznan Univ Tech, Inst Comp Sci, Poznan, PolandKatarzyna J. Purzycka - Institute of Bioorganic Chemistry, Polish Academy of Sciences
- Resource Type
- Journal article
- Publication Details
- Current genetics, Vol.62(2), pp.321-329
- DOI
- 10.1007/s00294-015-0550-6
- PMID
- 26650614
- PMCID
- PMC4826814
- NLM abbreviation
- Curr Genet
- ISSN
- 0172-8083
- eISSN
- 1432-0983
- Publisher
- Springer Nature
- Number of pages
- 9
- Grant note
- R01GM095622 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 1011RH25213 / National Science Foundation; National Science Foundation (NSF) GM095622 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA HOMING PLUS/2012-6/12 / Foundation for Polish Science; European Commission 2011/01/D/NZ1/03478; 2012/06/A/ST6/00384 / National Science Center, Poland; National Science Centre, Poland 0492/IP1/2013/72 / Ministry of Science and Higher Education, Poland University of Georgia Research Foundation
- Language
- English
- Date published
- 05/01/2016
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297429902771
Metrics
23 Record Views