Journal article
A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life
PLoS biology, Vol.20(9), e3001772
09/2022
DOI: 10.1371/journal.pbio.3001772
PMCID: PMC9481166
PMID: 36067248
Abstract
Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.
Details
- Title: Subtitle
- A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life
- Creators
- Sheng-Yi WuYurong WenNelson B. C SerreCathrine Charlotte Heiede LaursenAndrea Grostøl DietzBrian R TaylorMikhail DrobizhevRosana S MolinaAbhi AggarwalVladimir RancicMichael BeckerKlaus BallanyiKaspar PodgorskiHajime HiraseMaiken NedergaardMatyáš FendrychM. Joanne LemieuxDaniel F EberlAlan R KayRobert E CampbellYi Shen
- Resource Type
- Journal article
- Publication Details
- PLoS biology, Vol.20(9), e3001772
- DOI
- 10.1371/journal.pbio.3001772
- PMID
- 36067248
- PMCID
- PMC9481166
- NLM abbreviation
- PLoS Biol
- ISSN
- 1545-7885
- eISSN
- 1545-7885
- Publisher
- Public Library of Science
- Grant note
- DOI: 10.13039/501100000024, name: Canadian Institutes of Health Research, award: FS-154310; DOI: 10.13039/501100000038, name: Natural Sciences and Engineering Research Council of Canada, award: RGPIN 2018-04364; DOI: 10.13039/501100000038, name: Natural Sciences and Engineering Research Council of Canada, award: RGPIN-2016-06478; DOI: 10.13039/501100000038, name: Natural Sciences and Engineering Research Council of Canada, award: RGPIN-2020-05514; DOI: 10.13039/501100000038, name: Natural Sciences and Engineering Research Council of Canada, award: Graduate Scholarship; DOI: 10.13039/501100000146, name: Alberta Innovates - Technology Futures, award: Graduate Scholarship; DOI: 10.13039/501100000190, name: University of Alberta; DOI: 10.13039/501100000263, name: Parkinson Society Canada, award: Alberta Parkinson Society Fellowship; DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: No. 31870132 and No. 82072237; name: European Research Council, award: Grant No. 803048; name: Charles University Primus, award: Grant No. PRIMUS/19/SCI/09; DOI: 10.13039/501100009708, name: Novo Nordisk Fonden, award: NNF19OC0058058; DOI: 10.13039/501100009708, name: Novo Nordisk Fonden, award: NNF13OC0004258; DOI: 10.13039/501100003554, name: Lundbeckfonden, award: R155-2016-552; DOI: 10.13039/501100003554, name: Lundbeckfonden, award: R263-2017-4062; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01GM095903; DOI: 10.13039/100000011, name: Howard Hughes Medical Institute; DOI: 10.13039/100000001, name: National Science Foundation, award: 2037828; DOI: 10.13039/100000002, name: National Institutes of Health, award: U24 NS109107 (Resource for Multiphoton Characterization of Genetically-Encoded Probes)
- Language
- English
- Date published
- 09/2022
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984378335002771
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