A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus

Lecia Pewe; Haixia Zhou; Jason Netland; Chandra Tangudu; Heidi Olivares; Lei Shi; Dwight Look; Thomas Gallagher; Stanley Perlman

doi:10.1128/JVI.79.17.11335-11342.2005

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A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus

Journal article

Open access

Peer reviewed

A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus

Lecia Pewe, Haixia Zhou, Jason Netland, Chandra Tangudu, Heidi Olivares, Lei Shi, Dwight Look, Thomas Gallagher and Stanley Perlman

Journal of virology, Vol.79(17), pp.11335-11342

09/2005

DOI: 10.1128/JVI.79.17.11335-11342.2005

PMCID: PMC1193615

PMID: 16103185

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Abstract

Most animal species that can be infected with the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) do not reproducibly develop clinical disease, hindering studies of pathogenesis. To develop an alternative system for the study of SARS-CoV, we introduced individual SARS-CoV genes (open reading frames [ORFs]) into the genome of an attenuated murine coronavirus. One protein, the product of SARS-CoV ORF6, converted a sublethal infection to a uniformly lethal encephalitis and enhanced virus growth in tissue culture cells, indicating that SARS-CoV proteins function in the context of a heterologous coronavirus infection. Furthermore, these results suggest that the attenuated murine coronavirus lacks a virulence gene residing in SARS-CoV. Recombinant murine coronaviruses cause a reproducible and well-characterized clinical disease, offer virtually no risk to laboratory personnel, and should be useful for elucidating the role of SARS-CoV nonstructural proteins in viral replication and pathogenesis.

Cell Line

Reassortant Viruses - pathogenicity

Encephalitis - virology

Humans

Mice, Inbred C57BL

Virulence

Viral Nonstructural Proteins - genetics

Murine hepatitis virus - pathogenicity

Open Reading Frames - genetics

SARS Virus - chemistry

SARS Virus - pathogenicity

Animals

Coronavirus Infections - virology

Virus Replication

Viral Nonstructural Proteins - metabolism

Mice

SARS Virus - physiology

Murine hepatitis virus - genetics

Disease Models, Animal

Details

Title: Subtitle: A severe acute respiratory syndrome-associated coronavirus-specific protein enhances virulence of an attenuated murine coronavirus
Creators: Lecia Pewe - Department of Pediatrics, University of Iowa, Medical Laboratories 2042, Iowa City, 52242, USA
Haixia Zhou
Jason Netland
Chandra Tangudu
Heidi Olivares
Lei Shi
Dwight Look
Thomas Gallagher
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.79(17), pp.11335-11342
DOI: 10.1128/JVI.79.17.11335-11342.2005
PMID: 16103185
PMCID: PMC1193615
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: P01 AI060699 / NIAID NIH HHS
Language: English
Date published: 09/2005
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777474702771

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