A single valine residue plays an essential role in peripherin/rds targeting to photoreceptor outer segments

Raquel Y Salinas; Sheila A Baker; Sidney M Gospe III; Vadim Y Arshavsky

doi:10.1371/journal.pone.0054292

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A single valine residue plays an essential role in peripherin/rds targeting to photoreceptor outer segments

Journal article

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Peer reviewed

A single valine residue plays an essential role in peripherin/rds targeting to photoreceptor outer segments

Raquel Y Salinas, Sheila A Baker, Sidney M Gospe III and Vadim Y Arshavsky

PloS one, Vol.8(1), pp.e54292-e54292

2013

DOI: 10.1371/journal.pone.0054292

PMCID: PMC3544770

PMID: 23342122

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Abstract

Peripherin/retinal degeneration slow (rds) is an integral membrane protein specifically localized to the light-sensing organelle of the photoreceptor cell, the outer segment. Within the outer segment, peripherin is found at the edges of photoreceptor discs, where it plays a critical role in disc morphogenesis and maintenance. Peripherin loss or mutations are often associated with severe forms of visual impairments. Like all other resident outer segment proteins, peripherin is synthesized in the photoreceptor cell body and subsequently transported to the outer segment. In an effort to further examine peripherin's delivery to outer segments, we undertook a careful examination of its targeting sequence. Using a fluorescently labeled reporter expressed in the rods of transgenic tadpoles, we narrowed peripherin's targeting sequence to ten amino acids within its C-terminal tail. This small stretch of amino acid residues is both necessary and sufficient for outer segment targeting. We also conducted alanine scanning of all residues within this sequence and found that only a single residue, valine at position 332, is essential for outer segment targeting. This valine is conserved in all species and its mutation is sufficient to completely abrogate the targeting of full-length peripherin in mouse rods.

Peripherins

Membrane Glycoproteins - metabolism

Animals, Genetically Modified

Membrane Glycoproteins - chemistry

Structure-Activity Relationship

Nerve Tissue Proteins - genetics

Membrane Glycoproteins - genetics

Anura

Nerve Tissue Proteins - metabolism

Nerve Tissue Proteins - chemistry

Animals

Larva

Valine

Photoreceptor Cells - metabolism

Intermediate Filament Proteins - genetics

Mice

Intermediate Filament Proteins - metabolism

Intermediate Filament Proteins - chemistry

Details

Title: Subtitle: A single valine residue plays an essential role in peripherin/rds targeting to photoreceptor outer segments
Creators: Raquel Y Salinas - Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States of America
Sheila A Baker
Sidney M Gospe III
Vadim Y Arshavsky
Resource Type: Journal article
Publication Details: PloS one, Vol.8(1), pp.e54292-e54292
DOI: 10.1371/journal.pone.0054292
PMID: 23342122
PMCID: PMC3544770
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: T32 GM007184 / NIGMS NIH HHS EY12859 / NEI NIH HHS F31 EY022862 / NEI NIH HHS R01 EY012859 / NEI NIH HHS P30 EY005722 / NEI NIH HHS EY05722 / NEI NIH HHS
Language: English
Date published: 2013
Academic Unit: Iowa Neuroscience Institute; Biochemistry and Molecular Biology; University College Courses; Ophthalmology and Visual Sciences
Record Identifier: 9984024404202771

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