Journal article
A skeleton-tree-based approach to acinar morphometric analysis using microcomputed tomography with comparison of acini in young and old C57BL/6 mice
Journal of applied physiology (1985), Vol.120(12), pp.1402-1409
06/15/2016
DOI: 10.1152/japplphysiol.00923.2015
PMCID: PMC4909836
PMID: 26940656
Abstract
We seek to establish a method using interior tomographic techniques (Xradia MicroXCT-400) for acinar morphometric analysis using the pathway center lines from micro X-ray computed tomographic (Micro-CT) images as the road map. Through the application of these techniques, we present a method to extend the atlas of murine lungs to acinar levels and present a comparison between two age groups of the C57BL/6 strain. Lungs fixed via vascular perfusion were scanned using high-resolution Micro-CT protocols. Individual acini were segmented, and skeletonized paths to alveolar sacs from the entrance to the acinus were formed. Morphometric parameters, including branch lengths, diameters, and branching angles, were generated. Six mice each, at two age groups (∼20 and ∼90 wk of age), were studied. Additive Gaussian noise (0 mean and SD 1, 2, 5, and 10) was used to test the robustness of the analytical method. Noise-based variations were within ±6 μm for branch lengths and ±5 μm for diameters. At a noise level of 10, errors increased. Branch diameters were less susceptible to noise than lengths. There was >95% center line overlap across all noise levels. The measurements obtained using the center lines as a road map were not affected by added noise. Acini from younger mice had smaller branch diameters and lengths at all generations without significant differences in branching angles. The relative distribution of volume in the alveolar ducts was similar across both age groups. The method has been demonstrated to be repeatable and robust to image noise and provides a new, nondestructive technique to assess and compare acinar morphometry quantitatively.
Details
- Title: Subtitle
- A skeleton-tree-based approach to acinar morphometric analysis using microcomputed tomography with comparison of acini in young and old C57BL/6 mice
- Creators
- Abhilash S Kizhakke Puliyakote - Department of Radiology, University of Iowa, Iowa City, Iowa; Department of Biomedical Engineering, University of Iowa, Iowa City, IowaDragoş M Vasilescu - Department of Radiology, University of Iowa, Iowa City, IowaKriti Sen Sharma - Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia; andGe Wang - Department of Electrical and Computer Engineering, Virginia Polytechnic Institute and State University, Blacksburg, Virginia; and Department of Biomedical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VirginiaEric A Hoffman - Department of Radiology, University of Iowa, Iowa City, Iowa; Department of Biomedical Engineering, University of Iowa, Iowa City, Iowa; Department of Medicine, University of Iowa, Iowa City, Iowa; eric-hoffman@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.120(12), pp.1402-1409
- Publisher
- United States
- DOI
- 10.1152/japplphysiol.00923.2015
- PMID
- 26940656
- PMCID
- PMC4909836
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Grant note
- P30 ES005605 / NIEHS NIH HHS S10 OD018503 / NIH HHS R01 HL112986 / NHLBI NIH HHS R01 HL080285 / NHLBI NIH HHS
- Language
- English
- Date published
- 06/15/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Internal Medicine
- Record Identifier
- 9984051559202771
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