A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression

Steven H Green; Rebecca M Glover; Penny A Krumm; Timothy R Winters; Dianne L Atkins

doi:10.1016/S0008-6363(98)00217-X

Back

A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression

Journal article

Open access

Peer reviewed

A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression

Steven H Green, Rebecca M Glover, Penny A Krumm, Timothy R Winters and Dianne L Atkins

Cardiovascular research, Vol.41(1), pp.200-211

01/1999

DOI: 10.1016/S0008-6363(98)00217-X

PMID: 10325967

Files and links (1)

url

https://doi.org/10.1016/S0008-6363(98)00217-XView

Published (Version of record) Open Access

Abstract

The purpose of this investigation was to establish a model system to facilitate identification of the sympathetic neuronal factor(s) that promotes improved contractility in neonatal cardiac myocytes. Conditioned medium from PC12 cells with sympathetic phenotype served as the source of the neuronal factor. Contraction frequency, amplitude and velocity of cultured neonatal rat cardiac myocytes were measured by online video analysis. Interventions included in vitro sympathetic innervation, exposure to PC12 conditioned medium, neurotransmitters and antagonists. Metabolic activity was assayed by 2-deoxyglucose uptake. Troponin T isoform expression was analyzed by SDS-polyacrylamide gel electrophoresis. Medium conditioned by neuronal PC12 cells induced contractility changes similar to those induced by in vitro sympathetic innervation. These effects of PC12 conditioned medium and innervation were not suppressed by adrenergic or muscarinic antagonists nor reproduced by neuropeptide Y or somatostatin. Neuronal PC12 conditioned medium but not chromaffin PC12 conditioned medium, increased metabolic activity of the myocytes as detected by [3H]-2-deoxyglucose, indicating that the effect was specific to the neuronal PC12 cells. The in vitro switch of troponin T isoform expression was not altered by exposure to PC12 conditioned medium. Increased contractile function induced by sympathetic innervation is reproduced by PC12 conditioned medium, but neither is mediated by sympathetic or muscarinic neurotransmitters. Troponin T isoform expression is not related to the contractility changes. This model system will allow identification of the factor(s).

Analysis of Variance

Animals

Atropine - pharmacology

Culture Media, Conditioned

Deoxyglucose - metabolism

Electrophoresis, Polyacrylamide Gel

Immunoblotting

Myocardial Contraction - drug effects

Myocardium - metabolism

Neuropeptide Y - pharmacology

Parasympatholytics - pharmacology

PC12 Cells

Prazosin - pharmacology

Propranolol - pharmacology

Protein Isoforms

Rats

Rats, Inbred WKY

Somatostatin - pharmacology

Sympathetic Nervous System - metabolism

Sympatholytics - pharmacology

Troponin T - metabolism

Details

Title: Subtitle: A soluble neuronal factor alters contractile function of ventricular myocytes without effect on troponin T isoform expression
Creators: Steven H Green - Department of Biological Sciences, University of Iowa, Iowa City 52242, USA
Rebecca M Glover
Penny A Krumm
Timothy R Winters
Dianne L Atkins
Resource Type: Journal article
Publication Details: Cardiovascular research, Vol.41(1), pp.200-211
DOI: 10.1016/S0008-6363(98)00217-X
PMID: 10325967
ISSN: 0008-6363
eISSN: 1755-3245
Grant note: R01-DC02961 / NIDCD NIH HHS R01-HL43173 / NHLBI NIH HHS DK25295 / NIDDK NIH HHS
Language: English
Date published: 01/1999
Academic Unit: Stead Family Department of Pediatrics; Biology; Otolaryngology
Record Identifier: 9984217426802771

Metrics

23 Record Views

2 Times Cited - Web of Science