A spatial and projection-based transcriptomic atlas of paraventricular hypothalamic cell types

We would like to thank Drs. Mark Andermann, Joel Geerling, and Clifford Saper, as well as the Lowell, Tsai, and Resch laboratories for helpful discussions; Alysia Berns, Jia Yu, and Yanfang Li for technical support; the BNORC Functional Genomics and Bioinformatics Core (P30DK046200) and the Iowa Institute for Human Genetics Genomics Division (IIHG, RRID: SCR_023422) for helpful discussions and technical assistance with sc/snRNA-seq; Zachary Niziolek and the Bauer Core Facility at Harvard University, the BIDMC Flow Cytometry Core, and Heath Vignes, Michael Shey, and Thomas Kaufman of the Flow Cytometry Facility at the University of Iowa Carver College of Medicine for helpful discussions and technical support; the ICCB-Longwood Screening Facility of Harvard Medical School for assistance with the snRNA-seq experiments; Dr. Sayak Mitter and Vizgen support for technical assistance with the MERSCOPE platform; and Mara Jendro and Li-Chun (Queena) Lin for their assistance with MERSCOPE experiments within the Iowa NeuroBank Core in the Iowa Neuroscience Institute at the University of Iowa Carver College of Medicine. This research was funded by the following NIH grants to L.T.T.: R01DK128406; to B.B.L.: R01DK075632, R01DK134427, and R01DK096010; to J.M.R.: R00HL144923 and R01NS141072; and to M.C.M.: F31HL170784; T.C.B. and M.C.M. were supported by a pharmacological sciences predoctoral training grant T32GM144636. Additional funding to J.M.R. came from the American Heart Association (AHA 935362) , a University of Iowa Fraternal Order of Eagles Diabetes Research Center Pilot and Feasibility Catalyst Grant, and an Iowa Neuroscience Institute Early Stage Investigator award from the Carver Trust. Y.L. was supported by a predoctoral fellowship from the American Heart Association (AHA 25PRE1372983) . A.M.D. was supported by a postdoctoral fellowship from the Charles A. King Trust.