Journal article
A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures
Lab on a chip, Vol.15(21), pp.4166-4176
2015
DOI: 10.1039/C5LC00868A
PMCID: PMC4641750
PMID: 26338516
Abstract
The ability to generate stable, spatiotemporally controllable concentration gradients is critical for resolving the dynamics of cellular response to a chemical microenvironment. Here we demonstrate an acoustofluidic gradient generator based on acoustically oscillating sharp-edge structures, which facilitates in a step-wise fashion the rapid mixing of fluids to generate tunable, dynamic chemical gradients. By controlling the driving voltage of a piezoelectric transducer, we demonstrated that the chemical gradient profiles can be conveniently altered (spatially controllable). By adjusting the actuation time of the piezoelectric transducer, moreover, we generated pulsatile chemical gradients (temporally controllable). With these two characteristics combined, we have developed a spatiotemporally controllable gradient generator. The applicability and biocompatibility of our acoustofluidic gradient generator are validated by demonstrating the migration of human dermal microvascular endothelial cells (HMVEC-d) in response to a generated vascular endothelial growth factor (VEGF) gradient, and by preserving the viability of HMVEC-d cells after long-term exposure to an acoustic field. Our device features advantages such as simple fabrication and operation, compact and biocompatible device, and generation of spatiotemporally tunable gradients.
Details
- Title: Subtitle
- A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures
- Creators
- Po-Hsun Huang - Pennsylvania State UniversityChung Yu Chan - Pennsylvania State UniversityPeng Li - Pennsylvania State UniversityNitesh Nama - Pennsylvania State UniversityYuliang Xie - Pennsylvania State UniversityCheng-Hsin Wei - Pennsylvania State UniversityYuchao Chen - Pennsylvania State UniversityDaniel Ahmed - Pennsylvania State UniversityTony Jun Huang - Pennsylvania State University
- Resource Type
- Journal article
- Publication Details
- Lab on a chip, Vol.15(21), pp.4166-4176
- DOI
- 10.1039/C5LC00868A
- PMID
- 26338516
- PMCID
- PMC4641750
- NLM abbreviation
- Lab Chip
- ISSN
- 1473-0197
- eISSN
- 1473-0189
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation, award: CBET-1438126, IIP-1534645; DOI: 10.13039/100000002, name: National Institutes of Health, award: 1 R01 GM112048-01A1, 1R33EB019785-01
- Language
- English
- Date published
- 2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984197141802771
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