A test of insulator interactions in Drosophila

Emily J Kuhn; Michaela M Viering; Katherine M Rhodes; Pamela K Geyer

doi:10.1093/emboj/cdg241

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A test of insulator interactions in Drosophila

Journal article

Open access

Peer reviewed

A test of insulator interactions in Drosophila

Emily J Kuhn, Michaela M Viering, Katherine M Rhodes and Pamela K Geyer

The EMBO journal, Vol.22(10), pp.2463-2471

05/15/2003

DOI: 10.1093/emboj/cdg241

PMCID: PMC155999

PMID: 12743040

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Abstract

Insulators are a class of elements that define independent domains of gene function. The Drosophila gypsy insulator is proposed to establish regulatory isolation by forming loop domains that constrain interactions between transcriptional control elements. This supposition is based upon the observation that insertion of a single gypsy insulator between an enhancer and promoter blocks enhancer function, while insertion of two gypsy insulators promotes enhancer bypass and activation of transcription. To investigate this model, we determined whether non-gypsy insulators interacted with each other and with the gypsy insulator. Pairs of scs or scs' insulators blocked enhancer function. Further, an intervening scs insulator did not block gypsy insulator interactions. Taken together, these data suggest that not all Drosophila insulators interact, with this property restricted to some insulators, such as gypsy. Three gypsy insulators inserted between an enhancer and promoter blocked enhancer function, indicating that gypsy insulator interactions may be restricted to pairs. Our studies imply that formation of loop domains may represent one of many mechanisms used by insulators to impart regulatory isolation.

Insulator Elements - genetics

Phenotype

Animals

Drosophila melanogaster - physiology

Gene Expression Regulation

Molecular Sequence Data

Organisms, Genetically Modified

Genome

Nucleic Acid Conformation

Transgenes

Drosophila melanogaster - genetics

Details

Title: Subtitle: A test of insulator interactions in Drosophila
Creators: Emily J Kuhn - Molecular Biology Program and Department of Biochemistry, University of Iowa, College of Medicine, Iowa City 52242, USA
Michaela M Viering
Katherine M Rhodes
Pamela K Geyer
Resource Type: Journal article
Publication Details: The EMBO journal, Vol.22(10), pp.2463-2471
Publisher: England
DOI: 10.1093/emboj/cdg241
PMID: 12743040
PMCID: PMC155999
ISSN: 0261-4189
eISSN: 1460-2075
Grant note: R01 GM042539 / NIGMS NIH HHS GM42539 / NIGMS NIH HHS
Language: English
Date published: 05/15/2003
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984024409902771

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