Journal article
A test of the CoHR motif associated with meiotic double-strand breaks in Saccharomyces cerevisiae
EMBO reports, Vol.5(1), pp.41-46
01/2004
DOI: 10.1038/sj.embor.7400050
PMCID: PMC1298961
PMID: 14710185
Abstract
Meiotic recombination is not random along chromosomes; rather, there are preferred regions for initiation called hotspots. Although the general properties of meiotic hotspots are known, the requirements at the DNA sequence level for the determination of hotspot activity are still unclear. The sequence of six known hotspots in Saccharomyces cerevisiae was compared to identify a common homology region (CoHR). They reported that the locations of CoHR sequences correspond to mapped double-strand break (DSB) sites along three chromosomes (I, III, VI). We report here that a deletion of CoHR at HIS2, a hotspot used to identify the motif, has no significant effect on recombination. In the absence of CoHR, DSB formation occurs at a high frequency and at the same sequences as in wild-type strains. In cases where the deletion of sequences containing the CoHR motif has been shown to reduce recombination, we propose that it may be a reflection of the location of the deletion, rather than the loss of CoHR, per se.
Details
- Title: Subtitle
- A test of the CoHR motif associated with meiotic double-strand breaks in Saccharomyces cerevisiae
- Creators
- Stuart J Haring - Department of Biological Sciences, University of Iowa, 204 Biology Building East, Iowa City, Iowa 52242, USALucas J LautnerJosep M ComeronRobert E Malone
- Resource Type
- Journal article
- Publication Details
- EMBO reports, Vol.5(1), pp.41-46
- Publisher
- England
- DOI
- 10.1038/sj.embor.7400050
- PMID
- 14710185
- PMCID
- PMC1298961
- ISSN
- 1469-221X
- eISSN
- 1469-3178
- Language
- English
- Date published
- 01/2004
- Academic Unit
- Biology; Internal Medicine
- Record Identifier
- 9983991996402771
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