Journal article
A transcriptomic map of murine and human alopecia areata
JCI insight, Vol.5(13), e137424
07/09/2020
DOI: 10.1172/jci.insight.137424
PMCID: PMC7406251
PMID: 32453712
Abstract
Alopecia areata (AA) is a common autoimmune condition, presenting initially with loss of hair without other overt skin changes. The unremarkable appearance of the skin surface contrasts with the complex immune activity occurring at the hair follicle. AA pathogenesis is due to the loss of immune privilege of the hair follicle, leading to autoimmune attack. Although the literature has focused on CD8(+) T cells, vital roles for CD4(+) T cells and antigen-presenting cells have been suggested. Here, we use single-cell sequencing to reveal distinct expression profiles of immune cells in murine AA. We found clonal expansions of both CD4(+) and CD8(+) T cells, with shared clonotypes across varied transcriptional states. The murine AA data were used to generate highly predictive models of human AA disease. Finally, single-cell sequencing of T cells in human AA recapitulated the clonotypic findings and the gene expression of the predictive models.
Details
- Title: Subtitle
- A transcriptomic map of murine and human alopecia areata
- Creators
- Nicholas Borcherding - Department of Pathology.Sydney B. Crotts - Department of Dermatology.Luana S. Ortolan - Univ Iowa, Coll Med, Dept Dermatol, Iowa City, IA 52242 USANicholas Henderson - Department of Dermatology.Nicholas L. Bormann - University of IowaAli Jabbari - Cancer Biology Graduate Program.
- Resource Type
- Journal article
- Publication Details
- JCI insight, Vol.5(13), e137424
- DOI
- 10.1172/jci.insight.137424
- PMID
- 32453712
- PMCID
- PMC7406251
- NLM abbreviation
- JCI Insight
- ISSN
- 2379-3708
- eISSN
- 2379-3708
- Publisher
- Amer Soc Clinical Investigation Inc
- Number of pages
- 14
- Grant note
- I01BX004907 / United States Department of Veterans Affairs, Biomedical Laboratory Research and Development Service; US Department of Veterans Affairs Carver College of Medicine AR069111; CA206255 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA University of Iowa Carver College of Medicine Iowa City Veterans Affairs Medical Center P30CA086862 / Holden Comprehensive Cancer Center (NCI of the NIH) P30CA086862 / National Cancer Institute (NCI) of the NIH Carver College of Medicine/Holden Comprehensive Cancer Center core research facility at the University of Iowa Holden Comprehensive Cancer Center
- Language
- English
- Date published
- 07/09/2020
- Academic Unit
- Dermatology
- Record Identifier
- 9984296306702771
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