Journal article
A transcriptomics approach uncovers novel roles for poly(ADP-ribosyl)ation in the basal defense response in Arabidopsis thaliana
PloS one, Vol.12(12), pp.e0190268-e0190268
2017
DOI: 10.1371/journal.pone.0190268
PMCID: PMC5746271
PMID: 29284022
Abstract
Pharmacological inhibition of poly(ADP-ribose) polymerase (PARP) or loss of Arabidopsis thaliana PARG1 (poly(ADP-ribose) glycohydrolase) disrupt a subset of plant defenses. In the present study we examined the impact of altered poly(ADP-ribosyl)ation on early gene expression induced by the microbe-associate molecular patterns (MAMPs) flagellin (flg22) and EF-Tu (elf18). Stringent statistical analyses and filtering identified 178 genes having MAMP-induced mRNA abundance patterns that were altered by either PARP inhibitor 3-aminobenzamide (3AB) or PARG1 knockout. From the identified set of 178 genes, over fifty Arabidopsis T-DNA insertion lines were chosen and screened for altered basal defense responses. Subtle alterations in callose deposition and/or seedling growth in response to those MAMPs were observed in knockouts of At3g55630 (FPGS3, a cytosolic folylpolyglutamate synthetase), At5g15660 (containing an F-box domain), At1g47370 (a TIR-X (Toll-Interleukin Receptor domain)), and At5g64060 (a predicted pectin methylesterase inhibitor). Over-represented GO terms for the gene expression study included "innate immune response" for elf18/parg1, highlighting a subset of elf18-activated defense-associated genes whose expression is altered in parg1 plants. The study also allowed a tightly controlled comparison of early mRNA abundance responses to flg22 and elf18 in wild-type Arabidopsis, which revealed many differences. The PARP inhibitor 3-methoxybenzamide (3MB) was also used in the gene expression profiling, but pleiotropic impacts of this inhibitor were observed. This transcriptomics study revealed targets for further dissection of MAMP-induced plant immune responses, impacts of PARP inhibitors, and the molecular mechanisms by which poly(ADP-ribosyl)ation regulates plant responses to MAMPs.
Details
- Title: Subtitle
- A transcriptomics approach uncovers novel roles for poly(ADP-ribosyl)ation in the basal defense response in Arabidopsis thaliana
- Creators
- Amy G Briggs - Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, United States of AmericaLori C Adams-Phillips - Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, United States of AmericaBrian D Keppler - Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, United States of AmericaSophia G Zebell - Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, United States of AmericaKyle C Arend - Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, United States of AmericaApril A Apfelbaum - Department of Biology, Beloit College, Beloit, WI, United States of AmericaJoshua A Smith - Department of Biology, Beloit College, Beloit, WI, United States of AmericaAndrew F Bent - Department of Plant Pathology, University of Wisconsin-Madison, Madison, WI, United States of America
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.12(12), pp.e0190268-e0190268
- DOI
- 10.1371/journal.pone.0190268
- PMID
- 29284022
- PMCID
- PMC5746271
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Grant note
- F32 GM075599 / NIGMS NIH HHS 5F32GM075599-02 / NIH HHS
- Language
- English
- Date published
- 2017
- Academic Unit
- Biology
- Record Identifier
- 9984217425102771
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