Journal article
A trial to prepare biodegradable collagen-hydroxyapatite composites for bone repair
Journal of biomaterials science. Polymer ed, Vol.12(6), pp.689-705
01/01/2001
DOI: 10.1163/156856201316883485
PMID: 11556744
Abstract
This paper is a trial to prepare collagen-hydroxyapatite composites in vitro by an alternate immersion method. Collagen sponges of different biodegradabilities were prepared through chemical cross-linking
of Type I collagen with glutaraldehyde (GA) at concentrations of 0.2, 1.0, and 2.0 wt%. The sponges were immersed at 37°C in Tris-HCl-buffered solution containing 200 mM CaCl
2
(pH 7.4) for
2 h and then in an aqueous solution of 120 mM Na
2
HPO
4
(pH 9.3) for a 2 h further (one immersion cycle). The alternate immersion cycle was repeated for different times to obtain collagen-hydroxyapatite
composites. The characterization of the resulting composites was performed by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The weight
of composites increased with an increase in immersion cycles and the rate of increase became greater with higher GA cross-linking levels for collagen sponge preparation. The pH of the phosphate solution
decreased with the immersion cycle, which suggests H
+
generation accompanied hydroxyapatite formation. Irrespective of the GA concentration and immersion cycle, every composite showed IR absorption
bands attributable to phosphate and hydroxyl groups at 950-1100 or 550-650 and 3000-3500 cm
-1
and broad peaks specific to hydroxyapatite on the XRD charts. SEM study revealed small white clusters
of hydroxyapatite interspersed uniformly on/in the collagen framework without any preferential orientation. The composite prepared from 0.2 wt% GA cross-linked collagen sponge which showed favourable characteristics
was applied to a rat skull defect to evaluate its osteoconductivity as well as biodegradability. The formation of new bone tissue was histologically observed at the defect 12 weeks after application in
marked contrast to the collagen sponge alone. The composite degraded without any inflammation reaction. It is concluded that the collagen-hydroxyapatite composite prepared by the present method is a biodegradable
biomaterial of osteoconductivity applicable to bone repair.
Details
- Title: Subtitle
- A trial to prepare biodegradable collagen-hydroxyapatite composites for bone repair
- Creators
- Annie John - Kyoto UniversityLiu Hong - Kyoto UniversityYoshito Ikada - Suzuka University of Medical ScienceYasuhiko Tabata - Kyoto University
- Resource Type
- Journal article
- Publication Details
- Journal of biomaterials science. Polymer ed, Vol.12(6), pp.689-705
- DOI
- 10.1163/156856201316883485
- PMID
- 11556744
- NLM abbreviation
- J Biomater Sci Polym Ed
- ISSN
- 0920-5063
- eISSN
- 1568-5624
- Publisher
- Taylor & Francis Group
- Language
- English
- Date published
- 01/01/2001
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984367726502771
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