Journal article
A two-component modular approach for enhancing T-cell activation utilizing a unique anti-FcγRI-streptavidin construct and microspheres coated with biotinylated-antigen
Biomolecular engineering, Vol.20(1), pp.21-33
2003
DOI: 10.1016/S1389-0344(02)00089-8
PMID: 12485681
Abstract
The professional antigen presenting cell (APC) plays an essential role in the initiation and propagation of the acquired immune response. Thus, much work has been done in designing strategies that target vaccine antigen (Ag) to APC. Utilizing recombinant DNA technology, we have created a unique two-component system that delivers biotinylated Ag to the Fc gamma receptor type I (FcγRI) on APC. Our studies demonstrate that we can successfully engineer FcγRI-specific targeting element proteins that simultaneously bind both biotin and recognize FcγRI. Additionally, we are able to engineer biotinylated Ag, which form functional elements when adsorbed onto latex microspheres. Furthermore, the targeting and functional element components bind to each other and successfully form two-component immunogens. T-cell activation in response to targeted Ag-laden microspheres is 10- to 100-fold greater than the response to the non-targeted Ag-laden microspheres. This enhancement is 100- to 1000-fold greater than the responses generated to soluble Ag. Thus, our results suggest that specific targeting of Ag-laden microspheres to FcγRI may significantly enhance the adjuvant properties of microparticulate delivery systems. Further development of this system may help to elucidate the mechanisms involved in generating enhanced responses to APC-targeted vaccines and significantly advance vaccine technology.
Details
- Title: Subtitle
- A two-component modular approach for enhancing T-cell activation utilizing a unique anti-FcγRI-streptavidin construct and microspheres coated with biotinylated-antigen
- Creators
- Mary C Walsh - Albany Medical College, Center for Immunology and Microbial Disease, MC-151, 47 New Scotland Avenue, Albany, NY 12201, USAJeffrey A Banas - Albany Medical Center HospitalStanley P Mudzinski - Albany Medical Center HospitalMark T Preissler - Albany Medical Center HospitalRobert F Graziano - MedarexEdmund J Gosselin - Albany Medical Center Hospital
- Resource Type
- Journal article
- Publication Details
- Biomolecular engineering, Vol.20(1), pp.21-33
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S1389-0344(02)00089-8
- PMID
- 12485681
- ISSN
- 1389-0344
- eISSN
- 1878-559X
- Language
- English
- Date published
- 2003
- Academic Unit
- Pediatric Dentistry; Dental Research
- Record Identifier
- 9984367755902771
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