A yeast two-hybrid screen for SYP-3 interactors identifies SYP-4, a component required for synaptonemal complex assembly and chiasma formation in Caenorhabditis elegans meiosis

Sarit Smolikov; Kristina Schild-Prüfert; Mónica P Colaiácovo

doi:10.1371/journal.pgen.1000669

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A yeast two-hybrid screen for SYP-3 interactors identifies SYP-4, a component required for synaptonemal complex assembly and chiasma formation in Caenorhabditis elegans meiosis

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A yeast two-hybrid screen for SYP-3 interactors identifies SYP-4, a component required for synaptonemal complex assembly and chiasma formation in Caenorhabditis elegans meiosis

Sarit Smolikov, Kristina Schild-Prüfert and Mónica P Colaiácovo

PLoS genetics, Vol.5(10), pp.e1000669-e1000669

10/2009

DOI: 10.1371/journal.pgen.1000669

PMCID: PMC2742731

PMID: 19798442

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Abstract

The proper assembly of the synaptonemal complex (SC) between homologs is critical to ensure accurate meiotic chromosome segregation. The SC is a meiotic tripartite structure present from yeast to humans, comprised of proteins assembled along the axes of the chromosomes and central region (CR) proteins that bridge the two chromosome axes. Here we identify SYP-4 as a novel structural component of the SC in Caenorhabditis elegans. SYP-4 interacts in a yeast two-hybrid assay with SYP-3, one of components of the CR of the SC, and is localized at the interface between homologs during meiosis. SYP-4 is essential for the localization of SYP-1, SYP-2, and SYP-3 CR proteins onto chromosomes, thereby playing a crucial role in the stabilization of pairing interactions between homologous chromosomes. In the absence of SYP-4, the levels of recombination intermediates, as indicated by RAD-51 foci, are elevated in mid-prophase nuclei, and crossover recombination events are significantly reduced. The lack of chiasmata observed in syp-4 mutants supports the elevated levels of chromosome nondisjunction manifested in high embryonic lethality. Altogether our findings place SYP-4 as a central player in SC formation and broaden our understanding of the structure of the SC and its assembly.

Caenorhabditis elegans - metabolism

Chromosomal Proteins, Non-Histone - metabolism

Caenorhabditis elegans - genetics

Chromatids - genetics

Caenorhabditis elegans Proteins - metabolism

Meiosis

Synaptonemal Complex - genetics

Chromosomal Proteins, Non-Histone - genetics

Synaptonemal Complex - metabolism

Chromatids - metabolism

Two-Hybrid System Techniques

Animals

Caenorhabditis elegans - cytology

Protein Binding

Caenorhabditis elegans Proteins - genetics

Details

Title: Subtitle: A yeast two-hybrid screen for SYP-3 interactors identifies SYP-4, a component required for synaptonemal complex assembly and chiasma formation in Caenorhabditis elegans meiosis
Creators: Sarit Smolikov - Harvard Medical School
Kristina Schild-Prüfert
Mónica P Colaiácovo
Resource Type: Journal article
Publication Details: PLoS genetics, Vol.5(10), pp.e1000669-e1000669
DOI: 10.1371/journal.pgen.1000669
PMID: 19798442
PMCID: PMC2742731
NLM abbreviation: PLoS Genet
ISSN: 1553-7390
eISSN: 1553-7404
Publisher: United States
Grant note: 01GM072551 / NIGMS NIH HHS\r\nR01 GM072551 / NIGMS NIH HHS
Language: English
Date published: 10/2009
Academic Unit: Biology
Record Identifier: 9983991997502771

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