Journal article
A β2 adrenergic receptor signaling complex assembled with the ca2+ channel Cav1.2
Science (American Association for the Advancement of Science), Vol.293(5527), pp.98-101
2001
DOI: 10.1126/science.293.5527.98
PMID: 11441182
Abstract
The existence of a large number of receptors coupled to heterotrimeric guanine nucleotide binding proteins (G proteins) raises the question of how a particular receptor selectively regulates specific targets. We provide insight into this question by identifying a prototypical macromolecular signaling complex. The β2adrenergic receptor was found to be directly associated with one of its ultimate effectors, the class C L-type calcium channel Cav1.2. This complex also contained a G protein, an adenylyl cyclase, cyclic adenosine monophosphate–dependent protein kinase, and the counterbalancing phosphatase PP2A. Our electrophysiological recordings from hippocampal neurons demonstrate highly localized signal transduction from the receptor to the channel. The assembly of this signaling complex provides a mechanism that ensures specific and rapid signaling by a G protein–coupled receptor.
Details
- Title: Subtitle
- A β2 adrenergic receptor signaling complex assembled with the ca2+ channel Cav1.2
- Creators
- Monika A Davare - Department of Pharmacology, University of Wisconsin, Madison, WI 53706, United StatesVladimir Avdonin - Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, United StatesDuane D Hall - University of Iowa, Internal MedicineErik M Peden - Department of Pharmacology, University of Wisconsin, Madison, WI 53706, United StatesAlain Burette - Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, NC 27599, United StatesRichard J Weinberg - Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, NC 27599, United StatesMary C Horne - Department of Pharmacology, University of Wisconsin, Madison, WI 53706, United StatesToshinori Hoshi - Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, United StatesJohannes W Hell - Department of Pharmacology, University of Wisconsin, Madison, WI 53706, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.293(5527), pp.98-101
- Publisher
- American Association for the Advancement of Science
- DOI
- 10.1126/science.293.5527.98
- PMID
- 11441182
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Language
- English
- Date published
- 2001
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984094386702771
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