Journal article
ADAM10 cleavage of N‐cadherin and regulation of cell–cell adhesion and β‐catenin nuclear signalling
The EMBO journal, Vol.24(4), pp.742-752
02/23/2005
DOI: 10.1038/sj.emboj.7600548
PMCID: PMC1142587
PMID: 15933724
Abstract
Cadherins are critically involved in tissue development and tissue homeostasis. We demonstrate here that neuronal cadherin (N‐cadherin) is cleaved specifically by the disintegrin and metalloproteinase ADAM10 in its ectodomain. ADAM10 is not only responsible for the constitutive, but also for the regulated, shedding of this adhesion molecule in fibroblasts and neuronal cells directly regulating the overall levels of N‐cadherin expression at the cell surface. The ADAM10‐induced N‐cadherin cleavage resulted in changes in the adhesive behaviour of cells and also in a dramatic redistribution of β‐catenin from the cell surface to the cytoplasmic pool, thereby influencing the expression of β‐catenin target genes. Our data therefore demonstrate a crucial role of ADAM10 in the regulation of cell–cell adhesion and on β‐catenin signalling, leading to the conclusion that this protease constitutes a central switch in the signalling pathway from N‐cadherin at the cell surface to β‐catenin/LEF‐1‐regulated gene expression in the nucleus.
Details
- Title: Subtitle
- ADAM10 cleavage of N‐cadherin and regulation of cell–cell adhesion and β‐catenin nuclear signalling
- Creators
- Karina Reiss - Biochemical Institute, Christian‐Albrechts‐University KielThorsten Maretzky - Biochemical Institute, Christian‐Albrechts‐University KielAndreas Ludwig - Biochemical Institute, Christian‐Albrechts‐University KielThomas Tousseyn - Department for Human Genetics, KU Leuven and Flanders Interuniversity Institute for Biotechnology (VIB‐4)Bart de Strooper - Department for Human Genetics, KU Leuven and Flanders Interuniversity Institute for Biotechnology (VIB‐4)Dieter Hartmann - Department for Human Genetics, KU Leuven and Flanders Interuniversity Institute for Biotechnology (VIB‐4)Paul Saftig - Biochemical Institute, Christian‐Albrechts‐University Kiel
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.24(4), pp.742-752
- Publisher
- John Wiley & Sons, Ltd
- DOI
- 10.1038/sj.emboj.7600548
- PMID
- 15933724
- PMCID
- PMC1142587
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Number of pages
- 11
- Language
- English
- Date published
- 02/23/2005
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984094339802771
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