Journal article
ALTERATIONS IN CONSTITUENT URINARY PROTEINS IN RESPONSE TO BLADDER OUTLET OBSTRUCTION IN RATS
The Journal of urology, Vol.159(5), pp.1747-1751
05/01/1998
DOI: 10.1097/00005392-199805000-00105
PMID: 9554405
Abstract
Purpose: Benign prostatic hyperplasia, resulting in bladder outflow obstruction, induces well recognized clinical symptoms and morphologic bladder changes. Despite these phenomenon, relatively little is known with regard to the precise molecular events occurring in the bladder as a consequence of obstruction. In an effort to screen for alterations in bladder gene expression induced by obstruction, and/or alterations in uroepithelial integrity, this study compared pre-and post-obstructive constituent urinary proteins in an animal model. Materials and Methods: Outlet obstruction was created using a previously established model system. Experimental animals were surgically obstructed for either 2 or 7 days, at which time the urine was aspirated and the bladders removed and weighed. Urinary proteins were separated using 2-D PAGE. Following comparison of sham versus experimental animals, microsequencing was performed on proteins that were down regulated. Results: Duplicate experiments confirmed the presence of outflow obstruction. Statistically significant increases (p [removed]
Details
- Title: Subtitle
- ALTERATIONS IN CONSTITUENT URINARY PROTEINS IN RESPONSE TO BLADDER OUTLET OBSTRUCTION IN RATS
- Creators
- JENNIFER A. Burns - University of IowaKARL J. Kreder - University of IowaDAVID M. Lubaroff - University of IowaWILLIAM A. See - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of urology, Vol.159(5), pp.1747-1751
- DOI
- 10.1097/00005392-199805000-00105
- PMID
- 9554405
- ISSN
- 0022-5347
- eISSN
- 1527-3792
- Language
- English
- Date published
- 05/01/1998
- Academic Unit
- Obstetrics and Gynecology; Urology
- Record Identifier
- 9984383911502771
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