Journal article
AMP-activated protein kinase kinase activity and phosphorylation of AMP-activated protein kinase in contracting muscle of sedentary and endurance-trained rats
American journal of physiology: endocrinology and metabolism, Vol.289(4), pp.E710-715
10/2005
DOI: 10.1152/ajpendo.00155.2005
PMID: 15928023
Abstract
This study was designed to examine activity of AMP-activated protein kinase kinase (AMPKK) in muscles from nontrained and endurance-trained rats. Rats were trained 5 days/wk, 2 h/day for 8 wk at a final intensity of 32 m/min up a 15% grade with 30-s sprints at 53 m/min every 10 min. Gastrocnemius muscles were stimulated in situ in trained and nontrained rats for 5 min at frequencies of 0.4/s and 1/s. Gastrocnemius LKB1 protein, a putative component of the AMPKK complex (LKB1, STRAD, and MO25), increased approximately twofold in response to training. Phosphorylation of AMP-activated protein kinase (AMPK) determined by Western blot and AMPK activity of immunoprecipitates (both isoforms) was increased at both stimulation rates in both trained and nontrained muscles. AMPKK activity was 73% lower in resuspended polyethylene glycol precipitates of muscle extracts from the trained compared with nontrained rats. AMPKK activity did not increase in either trained or nontrained muscle in response to electrical stimulation, even though phospho-AMPK did increase. These results suggest that AMPKK is activated during electrical stimulation of both trained and nontrained muscle by mechanisms other than covalent modification.
Details
- Title: Subtitle
- AMP-activated protein kinase kinase activity and phosphorylation of AMP-activated protein kinase in contracting muscle of sedentary and endurance-trained rats
- Creators
- Denise Hurst - Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT 84602, USAEric B TaylorTroy D ClineLyle J GreenwoodCori L ComptonJeremy D LambWilliam W Winder
- Resource Type
- Journal article
- Publication Details
- American journal of physiology: endocrinology and metabolism, Vol.289(4), pp.E710-715
- Publisher
- United States
- DOI
- 10.1152/ajpendo.00155.2005
- PMID
- 15928023
- ISSN
- 0193-1849
- eISSN
- 1522-1555
- Grant note
- AR-41438 / NIAMS NIH HHS
- Language
- English
- Date published
- 10/2005
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025322402771
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