ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Kathleen A Sluka; Rajan Radhakrishnan; Christopher J Benson; Jayasheel O Eshcol; Margaret P Price; Kazimierz Babinski; Katherine M Audette; David C Yeomans; Steven P Wilson

doi:10.1016/j.pain.2006.09.038

Back

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Journal article

Peer reviewed

ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation

Kathleen A Sluka, Rajan Radhakrishnan, Christopher J Benson, Jayasheel O Eshcol, Margaret P Price, Kazimierz Babinski, Katherine M Audette, David C Yeomans and Steven P Wilson

Pain (Amsterdam), Vol.129(1), pp.102-112

2007

DOI: 10.1016/j.pain.2006.09.038

PMCID: PMC1941845

PMID: 17134831

View Online

Abstract

Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat, hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3−/− and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3−/− mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/+ mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3−/− mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3−/− mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult.

Proton

Ion

Acid

Nociceptor

Pain

Carrageenan

Details

Title: Subtitle: ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation
Creators: Kathleen A Sluka - Graduate Program in Physical Therapy and Rehabilitation Science, 1-252 Medical Education Bldg., The University of Iowa, Iowa City, IA 52242, USA
Rajan Radhakrishnan - Graduate Program in Physical Therapy and Rehabilitation Science, 1-252 Medical Education Bldg., The University of Iowa, Iowa City, IA 52242, USA
Christopher J Benson - Neuroscience Graduate Program, The University of Iowa, Iowa City, IA 52242, USA
Jayasheel O Eshcol - Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USA
Margaret P Price - Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USA
Kazimierz Babinski - PainCeptor Pharma Corp., Montreal, Canada
Katherine M Audette - Neuroscience Graduate Program, The University of Iowa, Iowa City, IA 52242, USA
David C Yeomans - Department of Anesthesia, Pain Research Center, Stanford University, Stanford, CA 94305-5117, USA
Steven P Wilson - Department of Pharmacology, Physiology and Neuroscience, University of South Carolina School of Medicine, Columbia, SC 29208, USA
Resource Type: Journal article
Publication Details: Pain (Amsterdam), Vol.129(1), pp.102-112
DOI: 10.1016/j.pain.2006.09.038
PMID: 17134831
PMCID: PMC1941845
NLM abbreviation: Pain
ISSN: 0304-3959
eISSN: 1872-6623
Publisher: Ovid Technologies (Wolters Kluwer Health)
Language: English
Date published: 2007
Academic Unit: Iowa Neuroscience Institute; Cardiovascular Medicine; Nursing; Physical Therapy and Rehabilitation Science; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040257602771

Metrics

41 Record Views

152 Times Cited - Web of Science

See more details