ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors

Ruthrotha B. Selvi; Snehajyoti Chatterjee; Dinesh Jagadeesan; Piyush Chaturbedy; Bangalore Srinivas Suma; Muthusamy Eswaramoorthy; Tapas K. Kundu

doi:10.1186/1477-3155-10-35

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ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors

Journal article

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ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors

Ruthrotha B. Selvi, Snehajyoti Chatterjee, Dinesh Jagadeesan, Piyush Chaturbedy, Bangalore Srinivas Suma, Muthusamy Eswaramoorthy and Tapas K. Kundu

Journal of nanobiotechnology, Vol.10(1), pp.35-35

08/02/2012

DOI: 10.1186/1477-3155-10-35

PMCID: PMC3479219

PMID: 22857258

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Abstract

Background: Intrinsically fluorescent glucose derived carbon nanospheres (CSP) efficiently enter mammalian cells and also cross the blood brain barrier (BBB). However, the mechanistic details of CSP entry inside mammalian cells and its specificity are not known. Results: In this report, the biochemical and cellular mechanism of CSP entry into the living cell have been investigated. By employing confocal imaging we show that CSP entry into the mammalian cells is an ATP-dependent clathrin mediated endocytosis process. Zeta potential studies suggest that it has a strong preference for cells which possess high levels of glucose transporters such as the glial cells, thereby enabling it to target individual organs/tissues such as the brain with increased specificity. Conclusion: The endocytosis of Glucose derived CSP into mammalian cells is an ATP dependent process mediated by clathrin coated pits. CSPs utilize the surface functional groups to target cells containing glucose transporters on its membrane thereby implicating a potential application for specific targeting of the brain or cancer cells.

Biotechnology & Applied Microbiology

Life Sciences & Biomedicine

Nanoscience & Nanotechnology

Science & Technology

Science & Technology - Other Topics

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Title: Subtitle: ATP driven clathrin dependent entry of carbon nanospheres prefer cells with glucose receptors
Creators: Ruthrotha B. Selvi - Jawaharlal Nehru Centre for Advanced Scientific Research
Snehajyoti Chatterjee - Jawaharlal Nehru Centre for Advanced Scientific Research
Dinesh Jagadeesan - Jawaharlal Nehru Centre for Advanced Scientific Research
Piyush Chaturbedy - Jawaharlal Nehru Centre for Advanced Scientific Research
Bangalore Srinivas Suma - Jawaharlal Nehru Centre for Advanced Scientific Research
Muthusamy Eswaramoorthy - Jawaharlal Nehru Centre for Advanced Scientific Research
Tapas K. Kundu - Jawaharlal Nehru Centre for Advanced Scientific Research
Resource Type: Journal article
Publication Details: Journal of nanobiotechnology, Vol.10(1), pp.35-35
DOI: 10.1186/1477-3155-10-35
PMID: 22857258
PMCID: PMC3479219
NLM abbreviation: J Nanobiotechnology
ISSN: 1477-3155
eISSN: 1477-3155
Publisher: Springer Nature
Number of pages: 9
Grant note: JNCASR; Department of Science & Technology (India) Sir JC Bose national fellowship, Department of Science and Technology, Govt. of India DBT/CSH/GIA/1752 / Department of Biotechnology, Government of India; Department of Biotechnology (DBT) India
Language: English
Date published: 08/02/2012
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984303853502771

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