Journal article
Aberrant Community Architecture and Attenuated Persistence of Uropathogenic Escherichia coli in the Absence of Individual IHF Subunits
PloS one, Vol.7(10), pp.e48349-e48349
10/25/2012
DOI: 10.1371/journal.pone.0048349
PMCID: PMC3485042
PMID: 23133584
Abstract
Uropathogenic Escherichia coli (UPEC) utilizes a complex community-based developmental pathway for growth within superficial epithelial cells of the bladder during cystitis. Extracellular DNA (eDNA) is a common matrix component of organized bacterial communities. Integration host factor (IHF) is a heterodimeric protein that binds to double-stranded DNA and produces a hairpin bend. IHF-dependent DNA architectural changes act both intrabacterially and extrabacterially to regulate gene expression and community stability, respectively. We demonstrate that both IHF subunits are required for efficient colonization of the bladder, but are dispensable for early colonization of the kidney. The community architecture of the intracellular bacterial communities (IBCs) is quantitatively different in the absence of either IhfA or IhfB in the murine model for human urinary tract infection (UTI). Restoration of Type 1 pili by ectopic production does not restore colonization in the absence of IhfA, but partially compensates in the absence of IhfB. Furthermore, we describe a binding site for IHF that is upstream of the operon that encodes for the P-pilus. Taken together, these data suggest that both IHF and its constituent subunits (independent of the heterodimer), are able to participate in multiple aspects of the UPEC pathogenic lifestyle, and may have utility as a target for treatment of bacterial cystitis.
Details
- Title: Subtitle
- Aberrant Community Architecture and Attenuated Persistence of Uropathogenic Escherichia coli in the Absence of Individual IHF Subunits
- Creators
- Sheryl S. Justice - The Ohio State UniversityBirong Li - Nationwide Children's HospitalJennifer S. Downey - Univ So Calif, Herman Ostrow Sch Dent, Div Biomed Sci, Los Angeles, CA USAShareef M. Dabdoub - Nationwide Children's HospitalM. Elizabeth Brockson - Nationwide Children's HospitalG. Duane Probst - Nationwide Childrens Hosp, Res Inst, Ctr Microbial Pathogenesis, Columbus, OH USAWilliam C. Ray - Nationwide Childrens Hosp, Res Inst, Battelle Ctr Math Med, Columbus, OH USASteven D. Goodman - Univ So Calif, Herman Ostrow Sch Dent, Div Biomed Sci, Los Angeles, CA USA
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.7(10), pp.e48349-e48349
- DOI
- 10.1371/journal.pone.0048349
- PMID
- 23133584
- PMCID
- PMC3485042
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library Science
- Number of pages
- 11
- Grant note
- 1R01DC011818-01 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01DC011818 / NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD)
- Language
- English
- Date published
- 10/25/2012
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984367653102771
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