Journal article
Aberrant Virion Assembly and Limited Glycoprotein C Production in Varicella-Zoster Virus-Infected Neurons
Journal of virology, Vol.87(17), pp.9643-9648
09/2013
DOI: 10.1128/JVI.01506-13
PMCID: PMC3754126
PMID: 23804641
Abstract
Highly pure (>95%) terminally differentiated neurons derived from pluripotent stem cells appear healthy at 2 weeks after infection with varicella-zoster virus (VZV), and the cell culture medium contains no infectious virus. Analysis of the healthy-appearing neurons revealed VZV DNA, transcripts, and proteins corresponding to the VZV immediate early, early, and late kinetic phases of replication. Herein, we further characterized virus in these neuronal cells, focusing on (i) transcription and expression of late VZV glycoprotein C (gC) open reading frame 14 (ORF14) and (ii) ultrastructural features of virus particles in neurons. The analysis showed that gC was not expressed in most infected neurons and gC expression was markedly reduced in a minority of VZV-infected neurons. In contrast, expression of the early-late VZV gE glycoprotein (ORF68) was abundant. Transcript analysis also showed decreased gC transcription compared with gE. Examination of viral structure by high-resolution transmission electron microscopy revealed fewer viral particles than typically observed in cells productively infected with VZV. Furthermore, viral particles were more aberrant, in that most capsids in the nuclei lacked a dense core and most enveloped particles in the cytoplasm were light particles (envelopes without capsids). Together, these results suggest a considerable deficiency in late-phase replication and viral assembly during VZV infection of neurons in culture.
Details
- Title: Subtitle
- Aberrant Virion Assembly and Limited Glycoprotein C Production in Varicella-Zoster Virus-Infected Neurons
- Creators
- Charles Grose - Children's Hospital, University of Iowa, Iowa City, Iowa, USAXiaoli Yu - Departments of NeurologyRandall J Cohrs - Departments of NeurologyJohn E Carpenter - Children's Hospital, University of Iowa, Iowa City, Iowa, USAJacqueline L Bowlin - Departments of NeurologyDon Gilden - Departments of Neurology
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.87(17), pp.9643-9648
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/JVI.01506-13
- PMID
- 23804641
- PMCID
- PMC3754126
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 09/2013
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093351502771
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