Aberrant water homeostasis detected by stable isotope analysis

Shannon P O'Grady; Adam R Wende; Christopher H Remien; Luciano O Valenzuela; Lindsey E Enright; Lesley A Chesson; E Dale Abel; Thure E Cerling; James R Ehleringer

doi:10.1371/journal.pone.0011699

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Aberrant water homeostasis detected by stable isotope analysis

Journal article

Open access

Peer reviewed

Aberrant water homeostasis detected by stable isotope analysis

Shannon P O'Grady, Adam R Wende, Christopher H Remien, Luciano O Valenzuela, Lindsey E Enright, Lesley A Chesson, E Dale Abel, Thure E Cerling and James R Ehleringer

PloS one, Vol.5(7), pp.e11699-e11699

07/21/2010

DOI: 10.1371/journal.pone.0011699

PMCID: PMC2908146

PMID: 20657736

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Abstract

While isotopes are frequently used as tracers in investigations of disease physiology (i.e., 14C labeled glucose), few studies have examined the impact that disease, and disease-related alterations in metabolism, may have on stable isotope ratios at natural abundance levels. The isotopic composition of body water is heavily influenced by water metabolism and dietary patterns and may provide a platform for disease detection. By utilizing a model of streptozotocin (STZ)-induced diabetes as an index case of aberrant water homeostasis, we demonstrate that untreated diabetes mellitus results in distinct combinations, or signatures, of the hydrogen (delta2H) and oxygen (delta18O) isotope ratios in body water. Additionally, we show that the delta2H and delta18O values of body water are correlated with increased water flux, suggesting altered blood osmolality, due to hyperglycemia, as the mechanism behind this correlation. Further, we present a mathematical model describing the impact of water flux on the isotopic composition of body water and compare model predicted values with actual values. These data highlight the importance of factors such as water flux and energy expenditure on predictive models of body water and additionally provide a framework for using naturally occurring stable isotope ratios to monitor diseases that impact water homeostasis.

Models, Theoretical

Body Water - metabolism

Animals

Deuterium - metabolism

Oxygen Isotopes - metabolism

Mice

Diabetes Mellitus, Experimental - metabolism

Details

Title: Subtitle: Aberrant water homeostasis detected by stable isotope analysis
Creators: Shannon P O'Grady - Department of Biology, University of Utah, Salt Lake City, Utah, United States of America. ogrady@biology.utah.edu
Adam R Wende
Christopher H Remien
Luciano O Valenzuela
Lindsey E Enright
Lesley A Chesson
E Dale Abel
Thure E Cerling
James R Ehleringer
Resource Type: Journal article
Publication Details: PloS one, Vol.5(7), pp.e11699-e11699
DOI: 10.1371/journal.pone.0011699
PMID: 20657736
PMCID: PMC2908146
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: U01 HL087947 / NHLBI NIH HHS U01HL087947 / NHLBI NIH HHS
Language: English
Date published: 07/21/2010
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984024528902771

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