Absence of cross-reactivity between murine Ly-6C and Ly-6G

Sanjai Nagendra; Annette J Schlueter

doi:10.1002/cyto.a.20007

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Absence of cross-reactivity between murine Ly-6C and Ly-6G

Journal article

Open access

Peer reviewed

Absence of cross-reactivity between murine Ly-6C and Ly-6G

Sanjai Nagendra and Annette J Schlueter

Cytometry. Part A, Vol.58(2), pp.195-200

04/2004

DOI: 10.1002/cyto.a.20007

PMID: 15057973

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Abstract

The Ly-6 family has many members, including Ly-6C and Ly-6G. A previous study suggested that the anti-Ly-6G antibody, RB6-8C5, may react with Ly-6Chi murine bone marrow (BM) cells. This finding has been interpreted as cross-reactivity of RB6-8C5 with the Ly-6C antigen, and has been generalized to many hematopoietic cell types, using the terminology Ly-6G/C. The present study was undertaken to determine whether anti-Ly-6G antibodies truly cross-react with the Ly-6C antigen on multiple hematopoietic cell types. Splenocytes, thymocytes, and BM cells obtained from Ly-6.1 and Ly-6.2 strains of mice were stained with a variety of antibodies to Ly-6C and Ly-6G. Flow cytometric analysis was performed on these populations. Evaluation of anti-Ly-6C and anti-Ly-6G staining showed only Ly-6C expression and no Ly-6G expression on subsets of splenic T and B cells and thymocytes from Ly-6.1 and Ly-6.2 mice. Bone marrow cells were identified that express both Ly-6G and Ly-6C; no Ly-6G+Ly-6C- populations were seen. Multiple Ly-6C+ hematopoietic cell populations were identified that do not stain with anti-Ly-6G antibodies. This calls into question the use of the Ly-6G/C nomenclature and suggests that epitopes recognized by anti-Ly-6G antibodies should simply be designated Ly-6G.

Oligodeoxyribonucleotides

Spleen - immunology

Bone Marrow Cells - cytology

DNA - pharmacology

Thymus Gland - cytology

Spleen - cytology

Cross Reactions - immunology

Mice, Inbred Strains

CD8 Antigens - analysis

B-Lymphocytes - drug effects

Animals

Antigens, Ly - immunology

Spleen - metabolism

CD4 Antigens - analysis

Antigens, Ly - metabolism

Mice

Thymus Gland - metabolism

B-Lymphocytes - metabolism

Bone Marrow Cells - metabolism

Details

Title: Subtitle: Absence of cross-reactivity between murine Ly-6C and Ly-6G
Creators: Sanjai Nagendra - Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Annette J Schlueter
Resource Type: Journal article
Publication Details: Cytometry. Part A, Vol.58(2), pp.195-200
DOI: 10.1002/cyto.a.20007
PMID: 15057973
NLM abbreviation: Cytometry A
ISSN: 1552-4922
eISSN: 1552-4930
Publisher: United States
Language: English
Date published: 04/2004
Academic Unit: Pathology
Record Identifier: 9984047785202771

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