Journal article
Abundance of MMPs and Cysteine Cathepsins in Caries-affected Dentin
Journal of dental research, Vol.93(3), pp.269-274
03/2014
DOI: 10.1177/0022034513516979
PMID: 24356440
Abstract
Degradation of dentin matrix components within caries dentin has been correlated with the activity of host-derived proteases, such as matrix metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship has not been fully established, we hypothesized that the abundance of MMPs and CTs in caries-affected dentin must be higher than in intact dentin. To test this premise, we obtained 5 slices (200 µm) from 5 intact teeth and from 5 caries-affected teeth (1 slice/tooth) and individually incubated them with primary antibodies for CT-B, CT-K, MMP-2, or MMP-9. Negative controls were incubated with pre-immune serum. Specimens were washed and re-incubated with the respective fluorescent secondary antibody. Collagen identification, attained by the autofluorescence capture technique, and protease localization were evaluated by multi-photon confocal microscopy. The images were analyzed with ZEN software, which also quantitatively measured the percentages of collagen and protease distribution in dentin compartments. The abundance of the test enzymes was markedly higher in caries-affected than in intact dentin. CT-B exhibited the highest percentage of co-localization with collagen, followed by MMP-9, MMP-2, and CT-K. The high expression of CTs and MMPs in caries-affected teeth indicates that those host-derived enzymes are intensely involved with caries progression.
Details
- Title: Subtitle
- Abundance of MMPs and Cysteine Cathepsins in Caries-affected Dentin
- Creators
- C.M.P Vidal - Department of Restorative Dentistry, Dental Materials Area, Piracicaba Dental School, State University of Campinas, Piracicaba, BrazilL Tjäderhane - Institute of Dentistry, University of Oulu, Oulu, Finland, Oulu University Hospital, Oulu, FinlandP.M Scaffa - Department of Restorative Dentistry, Dental Materials Area, Piracicaba Dental School, State University of Campinas, Piracicaba, BrazilI.L Tersariol - Centro Interdisciplinar de Investigação Bioquímica, University of Mogi das Cruzes, Mogi das Cruzes, Brazil, Department of Biochemistry, Federal University of São Paulo, BrazilD Pashley - Department of Oral Biology, College of Dental Medicine, Georgia Regents University, Augusta, GA, USAH.B Nader - Department of Biochemistry, Federal University of São Paulo, BrazilF.D Nascimento - Biomaterials Research Group and Biotechnology Division, UNIBAN, São Paulo, BrazilM.R Carrilho - Biomaterials Research Group and Biotechnology Division, UNIBAN, São Paulo, Brazil, Schulich School of Medicine & Dentistry, Western University, London, ON, Canada
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.93(3), pp.269-274
- DOI
- 10.1177/0022034513516979
- PMID
- 24356440
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Language
- English
- Date published
- 03/2014
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984065718302771
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