Journal article
Abundance ratio-dependent proteomic analysis by mass spectrometry
Analytical chemistry (Washington), Vol.75(4), pp.867-874
2003
DOI: 10.1021/ac026127j
PMID: 12622378
Abstract
The goal of quantitative proteomics is to determine the identity and relative quantity of each protein present in two or more complex protein samples. Here we describe a novel approach to quantitative proteomics. It is based on a highly accurate algorithm for the automated quantification of chromatographically fractionated, isotope-coded affinity-tagged peptides and MALDI quadrupole time-of-flight tandem mass spectrometry for their identification. The method is capable of detecting and selectively identifying those proteins within a complex mixture that show a difference in relative abundance. We demonstrate the effectiveness and the versatility of this approach in the analysis of a standard protein mixture, protein expression profiling in a human prostate cancer cell line model, and identification of the specific components of the multiprotein transcriptional machinery in S. cerevisiae.
Details
- Title: Subtitle
- Abundance ratio-dependent proteomic analysis by mass spectrometry
- Creators
- Timothy J GRIFFIN - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United StatesChris M LOCK - MDS-Sciex, 71 Four Valley Drive, Concord, Ontario, L4K 4V8, CanadaXiao-Jun LI - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United StatesAlpesh PATEL - Applied Biosystems, Foster City, California 94404, United StatesIryna CHERVETSOVA - MDS-Sciex, 71 Four Valley Drive, Concord, Ontario, L4K 4V8, CanadaHookeun LEE - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United StatesMichael E WRIGHT - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United StatesJeffrey A RANISH - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United StatesSharon S CHEN - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United StatesRuedi AEBERSOLD - Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103, United States
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.75(4), pp.867-874
- Publisher
- American Chemical Society; Washington, DC
- DOI
- 10.1021/ac026127j
- PMID
- 12622378
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Language
- English
- Date published
- 2003
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025323002771
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