Journal article
Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
Proceedings of the National Academy of Sciences - PNAS, Vol.97(7), pp.3094-3099
03/28/2000
DOI: 10.1073/pnas.97.7.3094
PMCID: PMC16198
PMID: 10725365
Abstract
The
Saccharomyces cerevisiae RAD30
gene functions in error-free replication of UV-damaged DNA.
RAD30
encodes a DNA polymerase, Pol η, which inserts two adenines opposite the two thymines of a
cis
-
syn
thymine–thymine (T–T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T–T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged DNA is nearly indistinguishable from that opposite nondamaged DNA, with frequencies of misincorporation of about 10
−2
to 10
−3
. These studies support the hypothesis that unlike most DNA polymerases, Pol η is able to tolerate distortions in DNA resulting from damage, which then enables the polymerase to utilize the intrinsic base pairing ability of the T–T dimer.
Details
- Title: Subtitle
- Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
- Creators
- M. Todd Washington - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061Robert E Johnson - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061Satya Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061Louise Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.97(7), pp.3094-3099
- DOI
- 10.1073/pnas.97.7.3094
- PMID
- 10725365
- PMCID
- PMC16198
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 03/28/2000
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024419102771
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