Journal article
Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
Proceedings of the National Academy of Sciences - PNAS, Vol.97(7), pp.3094-3099
03/28/2000
DOI: 10.1073/pnas.050491997
PMID: 10725365
Abstract
The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Pol η, which inserts two adenines opposite the two thymines of a cis-syn thymine-thymine (T-T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T-T dimer. Surprisingly, the accuracy of DNA synthesis opposite the damaged DNA is nearly indistinguishable from that opposite non-damaged DNA, with frequencies of misincorporation of about 10- 2 to 10-3. These studies support the hypothesis that unlike most DNA polymerases, Pol η is able to tolerate distortions in DNA resulting from damage, which then enables the polymerase to utilize the intrinsic base pairing ability of the T-T dimer.
Details
- Title: Subtitle
- Accuracy of thymine-thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase η
- Creators
- M. Todd Washington - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061Robert E Johnson - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061Satya Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061Louise Prakash - Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Medical Research Building, 11th & Mechanic Streets, Galveston, TX 77555-1061
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.97(7), pp.3094-3099
- DOI
- 10.1073/pnas.050491997
- PMID
- 10725365
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 03/28/2000
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024419102771
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