Journal article
Acetylcholine Synthesis and Accumulation in the CNS of Drosophila Larvae: Analysis of shibirets, a Mutant with a Temperature‐Sensitive Block in Synaptic Transmission
Journal of neurochemistry, Vol.40(5), pp.1386-1396
05/1983
DOI: 10.1111/j.1471-4159.1983.tb13581.x
PMID: 6300334
Abstract
: A radiochemical method is applied to the study of neurotransmitter metabolism in Drosophila. The larval CNS is a favorable system for analyzing acetylcholine (ACh) metabolism, since the pool of [3H]ACh rapidly reaches a steady state with a high ratio of intracellular [3H]ACh to [3H]choline. A temperature‐sensitive paralytic mutant, shibirets, shows reduced [3H]ACh accumulation at the restrictive temperature. This reduction is not the result of decreased synthesis of [3H]ACh, but rather an abnormally rapid rate of release, which is not prevented by blocking tetrodotoxin‐sensitive nerve activity.
Details
- Title: Subtitle
- Acetylcholine Synthesis and Accumulation in the CNS of Drosophila Larvae: Analysis of shibirets, a Mutant with a Temperature‐Sensitive Block in Synaptic Transmission
- Creators
- Chun‐Fang WuJames M BernekingDavid L Barker
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.40(5), pp.1386-1396
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- DOI
- 10.1111/j.1471-4159.1983.tb13581.x
- PMID
- 6300334
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Number of pages
- 11
- Language
- English
- Date published
- 05/1983
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070889602771
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