Acetylcholine-induced vasodilatation in rabbit hindlimb in vivo is not inhibited by analogues of L-arginine

Andreas Mügge; J. Antonio G Lopez; Donald J Piegors; Keith R Breese; Donald D Heistad

doi:10.1152/ajpheart.1991.260.1.H242

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Acetylcholine-induced vasodilatation in rabbit hindlimb in vivo is not inhibited by analogues of L-arginine

Journal article

Peer reviewed

Acetylcholine-induced vasodilatation in rabbit hindlimb in vivo is not inhibited by analogues of L-arginine

Andreas Mügge, J. Antonio G Lopez, Donald J Piegors, Keith R Breese and Donald D Heistad

The American journal of physiology, Vol.260(1 Pt 2), pp.H242-H247

01/1991

DOI: 10.1152/ajpheart.1991.260.1.H242

PMID: 1704195

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Abstract

Nitric oxide (NO) or related nitroso compounds are an endothelium-derived relaxing factor (EDRF), originating from metabolism of L-arginine, L-Arginine analogues with chemically altered guanidino moity are potent and specific inhibitors of EDRF(NO) release. We evaluated effects of two L-arginine analogues, NG-monomethyl-L-arginine (L-NMMA, 100 microM) and N omega-nitro-L-arginine (L-NARG, 30 microM), on acetylcholine-, substance P-, and nitroglycerin-induced relaxation in the blood-perfused rabbit hindlimb in vivo and femoral arteries in vitro. L-NMMA and L-NARG selectively inhibited the vasodilator response to acetylcholine in rabbit femoral arteries in vitro, whereas endothelium-independent response to nitroprusside increased. L-NMMA (1.6 mg/min ia) in the blood-perfused rabbit hindlimb in vivo increased vascular resistance in the hindlimb by 23 +/- 3% (means +/- SE; n = 10) but did not inhibit the vasodilator responses to acetylcholine or substance P. L-NARG (10 mg/kg iv) increased systemic blood pressure by 26 +/- 3% (n = 7) and vascular hindlimb resistance by 22 +/- 9% (n = 8), and blood flow to hindlimb musculature, measured with microspheres, decreased by 46 +/- 5% (n = 6). Pretreatment with L-NARG, however, did not impair vasodilator responses to acetylcholine and substance P. These findings are consistent with the view that basal tone in resistance vessels in the rabbit hindlimb may be mediated by nitroso compounds, whereas agonist-stimulated vasodilation may be mediated by other mechanisms that do not involve the NO-synthesizing enzyme.

Rabbits

Acetylcholine - pharmacology

Nitric Oxide - pharmacology

Hindlimb - physiology

Endothelium, Vascular - drug effects

Male

Substance P - pharmacology

omega-N-Methylarginine

Femoral Artery - drug effects

Nitroglycerin - pharmacology

Arginine - analogs & derivatives

Vascular Resistance - physiology

Endothelium, Vascular - physiology

Microspheres

Animals

Regional Blood Flow - drug effects

Hindlimb - blood supply

Arginine - pharmacology

Vascular Resistance - drug effects

Female

Femoral Artery - physiology

Nitroarginine

Vasodilation - drug effects

Vasodilation - physiology

Details

Title: Subtitle: Acetylcholine-induced vasodilatation in rabbit hindlimb in vivo is not inhibited by analogues of L-arginine
Creators: Andreas Mügge - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City
J. Antonio G Lopez
Donald J Piegors
Keith R Breese
Donald D Heistad
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.260(1 Pt 2), pp.H242-H247
Publisher: United States
DOI: 10.1152/ajpheart.1991.260.1.H242
PMID: 1704195
ISSN: 0002-9513
eISSN: 2163-5773
Grant note: NS-24621 / NINDS NIH HHS HL-14388 / NHLBI NIH HHS HL-14230 / NHLBI NIH HHS
Language: English
Date published: 01/1991
Academic Unit: Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040484202771

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