Journal article
Acetylcholine receptors in dissociated nucleus basalis of Meynert neurons of the rat
Neuroscience letters, Vol.130(2), pp.153-156
1991
DOI: 10.1016/0304-3940(91)90385-7
PMID: 1795874
Abstract
Electrical and pharmacological properties of acetylcholine (ACh)-induced currents in neurons dissociated from the nucleus basalis of Meynert (nBM) of immature (2-week-old) rats were investigated with the whole-cell mode of the patch-clamp technique. At a holding potential (
V
H) of −50 mV, ACh (10
−4M) evoked a transient inward current mimicked by nicotine (
I
nACh), followed by a sustained outward current mimicked by carbamylcholine (
I
mACh). The
K
D values were 1.2 × 10
−4 M for
I
nACh) and 8.7 × 10
−7 M for
I
mACh. The reversal potenial of
I
mACh was close to
E
K. The
I
mACh was determined to be elicited via the M
2 muscarinic receptor, based on the differences in sensitivity to muscarinic antagonists such as pirenzepine and AF-DX-116.
Details
- Title: Subtitle
- Acetylcholine receptors in dissociated nucleus basalis of Meynert neurons of the rat
- Creators
- Nobutoshi HarataNarito TateishiNorio Akaike
- Resource Type
- Journal article
- Publication Details
- Neuroscience letters, Vol.130(2), pp.153-156
- Publisher
- Elsevier Ireland Ltd
- DOI
- 10.1016/0304-3940(91)90385-7
- PMID
- 1795874
- ISSN
- 0304-3940
- eISSN
- 1872-7972
- Grant note
- DOI: 10.13039/501100001700, name: Ministry of Education, Culture, Sports, Science and Technology
- Language
- English
- Date published
- 1991
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025586202771
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