Journal article
Acid-Triggered Degradable Reagents for Differentiation of Adaptive and Innate Immune Responses to Leishmania-Associated Sugars
Angewandte Chemie (International ed.), Vol.54(33), pp.9610-9613
08/10/2015
DOI: 10.1002/anie.201502807
PMID: 26096941
Abstract
Lipopolysaccharides (LPS) of Leishmania spp are known to alter innate immune responses. However, the ability of these sugars to specifically alter adaptive T-cell responses is unclear. To study cap sugar-T-cell interactions, pathogen mimics (namely glycodendrimer-coated latex beads with acid-labile linkers) were synthesized. Upon lysosomal acidification, linker breakdown releases glycodendrimers for possible loading on antigen presenting molecules to induce T-cell growth. T-cell proliferation was indeed higher after macrophage exposure to mannobioside or -trioside-containing glycodendrimers than to non-functionalized beads. Yet, blocking phagolysosomal acidification only reduced T-cell proliferation with macrophages exposed to beads with an acid-labile-linker and not to covalently-linked beads. These sugar-modified reagents show that oligosaccharides alone can drive T-cell proliferation by acidification-requiring presentation, most significantly in NKT receptor (CD160)-restricted T cells.
Details
- Title: Subtitle
- Acid-Triggered Degradable Reagents for Differentiation of Adaptive and Innate Immune Responses to Leishmania-Associated Sugars
- Creators
- Rajarshi Roychoudhury - Department of Chemistry, Indiana University, Bloomington, IN 47405 (USA) http://www.indiana.edu/∼pohllab/Pedro A Martinez - Department of Epidemiology, University of Iowa, Iowa City, IA 52242 (USA)Tara Grinnage-Pulley - Department of Epidemiology, University of Iowa, Iowa City, IA 52242 (USA)Robert G Schaut - Department of Epidemiology, University of Iowa, Iowa City, IA 52242 (USA)Christine A Petersen - Department of Epidemiology, University of Iowa, Iowa City, IA 52242 (USA)Nicola L B Pohl - Department of Chemistry, Indiana University, Bloomington, IN 47405 (USA) http://www.indiana.edu/∼pohllab/. npohl@indiana.edu
- Resource Type
- Journal article
- Publication Details
- Angewandte Chemie (International ed.), Vol.54(33), pp.9610-9613
- DOI
- 10.1002/anie.201502807
- PMID
- 26096941
- NLM abbreviation
- Angew Chem Int Ed Engl
- ISSN
- 1433-7851
- eISSN
- 1521-3773
- Publisher
- Germany
- Language
- English
- Date published
- 08/10/2015
- Academic Unit
- Epidemiology; Internal Medicine
- Record Identifier
- 9983995145502771
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