Journal article
Acoustofluidic coating of particles and cells
Lab on a chip, Vol.16(22), pp.4366-4372
2016
DOI: 10.1039/C6LC00951D
PMCID: PMC5465870
PMID: 27754503
Abstract
On-chip microparticle and cell coating technologies enable a myriad of applications in chemistry, engineering, and medicine. Current microfluidic coating technologies often rely on magnetic labeling and concurrent deflection of particles across laminar streams of chemicals. Herein, we introduce an acoustofluidic approach for microparticle and cell coating by implementing tilted-angle standing surface acoustic waves (taSSAWs) into microchannels with multiple inlets. The primary acoustic radiation force generated by the taSSAW field was exploited in order to migrate the particles across the microchannel through multiple laminar streams, which contained the buffer and coating chemicals. We demonstrate effective coating of polystyrene microparticles and HeLa cells without the need for magnetic labelling. We characterized the coated particles and HeLa cells with fluorescence microscopy and scanning electron microscopy. Our acoustofluidic-based particle and cell coating method is label-free, biocompatible, and simple. It can be useful in the on-chip manufacturing of many functional particles and cells.
Details
- Title: Subtitle
- Acoustofluidic coating of particles and cells
- Creators
- Bugra Ayan - Pennsylvania State UniversityAdem Ozcelik - Duke UniversityHunter Bachman - Duke UniversityShi-Yang Tang - Pennsylvania State UniversityYuliang Xie - Pennsylvania State UniversityMengxi Wu - Duke UniversityPeng Li - Pennsylvania State UniversityTony Jun Huang - Pennsylvania State University
- Resource Type
- Journal article
- Publication Details
- Lab on a chip, Vol.16(22), pp.4366-4372
- DOI
- 10.1039/C6LC00951D
- PMID
- 27754503
- PMCID
- PMC5465870
- NLM abbreviation
- Lab Chip
- ISSN
- 1473-0197
- eISSN
- 1473-0189
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation, award: CBET-1438126, IDBR-1455658; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01 GM112048, R33 EB019785
- Language
- English
- Date published
- 2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984197134902771
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