Acquisition of manganous ions by mutans group streptococci

P D Bauer; C Trapp; D Drake; K G Taylor; R J Doyle

doi:10.1128/JB.175.3.819-825.1993

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Acquisition of manganous ions by mutans group streptococci

Journal article

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Peer reviewed

Acquisition of manganous ions by mutans group streptococci

P D Bauer, C Trapp, D Drake, K G Taylor and R J Doyle

Journal of bacteriology, Vol.175(3), pp.819-825

02/1993

DOI: 10.1128/JB.175.3.819-825.1993

PMCID: PMC196222

PMID: 8380803

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Abstract

The cariogenic bacteria Streptococcus sobrinus and S. cricetus were shown to have an absolute requirement for manganous ion in order to bind glucans or to adhere to glass in the presence of sucrose. The bacteria possessed a reasonably high affinity transport system for 54Mn2+, yielding a Km of about 12 microM. The Vmax for uptake of 54Mn2+ in S. sobrinus was increased when the bacteria were grown in Mn-depleted medium, but the Km remained the same. There was no evidence for two Mn2+ uptake systems, commonly observed for many bacteria. Ions such as Ca2+, Co2+, Co3+, Cu2+, Fe2+, Fe3+, Hg2+, Mg2+, Ni2+, and Zn2+ did not inhibit the uptake of 54Mn2+ by the bacteria, although Cd2+ was a potent inhibitor. Fractionation experiments showed that manganese was distributed in protoplasts (67%) and in the cell wall (33%). Approximately 80% of the 54Mn2+ in S. sobrinus was rapidly exchangeable with nonradioactive Mn2+. Electron spin resonance experiments showed that all of the manganese was bound or restricted in mobility. Proton motive force-dissipating agents increased the acquisition of 54Mn2+ by the streptococci, probably because the wall became more negatively charged when the cell could no longer produce protons.

Bacterial Adhesion

Biological Transport

Electron Spin Resonance Spectroscopy

Glucans - metabolism

Kinetics

Lectins - metabolism

Manganese - metabolism

Streptococcus sobrinus - drug effects

Streptococcus sobrinus - metabolism

Details

Title: Subtitle: Acquisition of manganous ions by mutans group streptococci
Creators: P D Bauer - University of Louisville
C Trapp - University of Louisville
D Drake - University of Louisville
K G Taylor
R J Doyle - University of Louisville
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.175(3), pp.819-825
DOI: 10.1128/JB.175.3.819-825.1993
PMID: 8380803
PMCID: PMC196222
ISSN: 0021-9193
eISSN: 1098-5530
Grant note: NIDR-07199 / PHS HHS
Language: English
Date published: 02/1993
Academic Unit: Endodontics; Dental Research
Record Identifier: 9984367648202771

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