Journal article
Actin and myosin dynamics are independent during Drosophila embryonic wound repair
Molecular biology of the cell, Vol.30(23), pp.2901-2912
11/01/2019
DOI: 10.1091/mbc.E18-11-0703
PMCID: PMC6822589
PMID: 31553671
Abstract
Collective cell movements play a central role in embryonic development, tissue repair, and metastatic disease. Cell movements are often coordinated by supracellular networks formed by the cytoskeletal protein actin and the molecular motor nonmuscle myosin II. During wound closure in the embryonic epidermis, the cells around the wound migrate collectively into the damaged region. In Drosophila embryos, mechanical tension stabilizes myosin at the wound edge, facilitating the assembly of a supracellular myosin cable around the wound that coordinates cell migration. Here, we show that actin is also stabilized at the wound edge. However, loss of tension or myosin activity does not affect the dynamics of actin at the wound margin. Conversely, pharmacological stabilization of actin does not affect myosin levels or dynamics around the wound. Together, our data suggest that actin and myosin are independently regulated during embryonic wound closure, thus conferring robustness to the embryonic wound healing response.
Details
- Title: Subtitle
- Actin and myosin dynamics are independent during Drosophila embryonic wound repair
- Creators
- Anna B. Kobb - University of TorontoKatheryn E. Rothenberg - University of TorontoRodrigo Fernandez-Gonzalez - University of Toronto
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.30(23), pp.2901-2912
- DOI
- 10.1091/mbc.E18-11-0703
- PMID
- 31553671
- PMCID
- PMC6822589
- NLM abbreviation
- Mol Biol Cell
- ISSN
- 1059-1524
- eISSN
- 1939-4586
- Publisher
- Amer Soc Cell Biology
- Number of pages
- 12
- Grant note
- Canada First Research Excellence Fund-University of Toronto Medicine by Design 156279 / Canadian Institutes of Health Research; Canadian Institutes of Health Research (CIHR) 418438-13 / Natural Sciences and Engineering Research Council of Canada; Natural Sciences and Engineering Research Council of Canada (NSERC); CGIAR ER14-10-170 / Ontario Ministry of Economic Development and Innovation CIHR; Canadian Institutes of Health Research (CIHR) Tier II Canada Research Chair in Quantitative Cell Biology and Morphogenesis 30279 / Canada Foundation for Innovation; CGIAR; Spanish Government
- Language
- English
- Date published
- 11/01/2019
- Academic Unit
- Biology
- Record Identifier
- 9984696708402771
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