Journal article
Action of α-Amanitin during Pyrophosphorolysis and Elongation by RNA Polymerase II
The Journal of biological chemistry, Vol.270(32), pp.19114-19119
08/11/1995
DOI: 10.1074/jbc.270.32.19114
PMID: 7642577
Abstract
Using defined elongation complexes formed on dC-tailed templates with Drosophila RNA polymerase II, we have examined elongation, pyrophosphorolysis, and DmS-II-mediated transcript cleavage and the inhibitory effect of alpha-amanitin on these processes. Analysis of pyrophosphorolysis on soluble or immobilized and templates confirmed that NTPs are liberated instead of dinucleotides that are released during DmS-II-mediated transcript cleavage. 10 microgram/ml alpha-amanitin completely inhibited DmS-II-mediated transcript cleavage but allowed extended pyrophosphorolysis and nucleotide addition to occur. alpha-Amanitin dramatically decreased the Vmax for nucleotide addition but only slightly affected the Km for nucleotides. Although the processes ae mechanistically distinct, both pyrophosphorolysis and DmS-II-mediated transcript cleavage frequently resulted in similar patterns of shortened transcript. Since polymerase molecules encounter similar kinetic barriers during both processes, it is possible that there is a common step in the reverse movement of the polymerase.
Details
- Title: Subtitle
- Action of α-Amanitin during Pyrophosphorolysis and Elongation by RNA Polymerase II
- Creators
- David R ChafinHongliang GuoDavid H Price
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.270(32), pp.19114-19119
- DOI
- 10.1074/jbc.270.32.19114
- PMID
- 7642577
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 08/11/1995
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984025294102771
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