Journal article
Actions of S-nitrosocysteine in the nucleus tractus solitarii are unrelated to release of nitric oxide
Brain research, Vol.746(1), pp.98-104
1997
DOI: 10.1016/S0006-8993(96)01188-2
PMID: 9037488
Abstract
Cardiovascular effects elicited by microinjection of
l-
S-nitrosocysteine in the nucleus tractus solitarii (NTS) were compared and contrasted with those produced by the dextroisomer, other nitric oxide donors and nitric oxide itself.
l-
S-nitrosocysteine produced dose-related decreases of arterial pressure and heart rate. In contrast,
d-
S-nitrosocysteine,
S-nitrosoglutathione, glyceryl trinitrate, and sodium nitroprusside produced minimal responses that were not dose-related. Likewise, injection of cystine and nitric oxide, two products of
S-nitrosocysteine breakdown, produced no significant response. Headspace analysis using chemiluminescence revealed that
l- and
d-
S-nitrosocysteine released identical amounts of nitric oxide when exposed to homogenates of whole rat brain. Responses to
l-
S-nitrosocysteine were not affected by local injection of oxyhemoglobin or the nitric oxide synthase inhibitor
l-nitroarginine methylester. Although injection of
l-cysteine into the NTS produced responses similar to those seen with injection of
l-
S-nitrosocysteine, blockade of excitatory amino acid receptors with kynurenic acid inhibited responses to cysteine but not those to the nitrosothiol. The study demonstrates that
S-nitrosocysteine is biologically active in the NTS. Its action is independent of release of nitric oxide from the nitrosothiol but may be mediated through stereoselective sites on target neurons.
Details
- Title: Subtitle
- Actions of S-nitrosocysteine in the nucleus tractus solitarii are unrelated to release of nitric oxide
- Creators
- Hisashi Ohta - Department of Neurology, University of Iowa and Department of Veterans Affairs Medical Center, Iowa City, IA 52242, USAJames N Bates - Department of Anesthesiology, University of Iowa, Iowa City, IA 52242, USAStephen J Lewis - Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USAWilliam T Talman - Department of Neurology, University of Iowa and Department of Veterans Affairs Medical Center, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Brain research, Vol.746(1), pp.98-104
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0006-8993(96)01188-2
- PMID
- 9037488
- ISSN
- 0006-8993
- eISSN
- 1872-6240
- Language
- English
- Date published
- 1997
- Academic Unit
- Neurology; Anesthesia
- Record Identifier
- 9984006428302771
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