Journal article
Activated B lymphocytes and tumor cell lysate as an effective cellular cancer vaccine
Cancer immunology, immunotherapy, Vol.70(11), pp.3093-3103
11/2021
DOI: 10.1007/s00262-021-02914-7
PMCID: PMC8720267
PMID: 33765210
Abstract
Cancer vaccines that utilize patient antigen-presenting cells to fight their own tumors have shown exciting promise in many preclinical studies, but have proven quite challenging to translate to clinical feasibility. Dendritic cells have typically been the cell of choice for such vaccine platforms, due to their ability to endocytose antigens nonspecifically, and their expression of multiple surface molecules that enhance antigen presentation. However, dendritic cells are present in low numbers in human peripheral blood and must be matured in culture before use in vaccines. Mature B lymphocytes, in contrast, are relatively abundant in peripheral blood, and can be quickly activated and expanded in overnight cultures. We devised an optimal stimulation cocktail that engages the B cell antigen receptor, CD40, TLR4 and TLR7, to activate B cells to present antigens from lysates of the recipient's tumor cells, precluding the need for known tumor antigens. This B cell vaccine (Bvac) improved overall survival from B16F1 melanoma challenge, as well as reduced tumor size and increased time to tumor appearance. Bvac upregulated B cell antigen presentation molecules, stimulated activation of both CD4
and CD8
T cells, and induced T cell migration. Bvac provides an alternative cellular vaccine strategy that has considerable practical advantages for translation to clinical settings.
Details
- Title: Subtitle
- Activated B lymphocytes and tumor cell lysate as an effective cellular cancer vaccine
- Creators
- Kyp L Oxley - University of IowaBrett M Hanson - University of IowaAshley N Zani - University of IowaGail A Bishop - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cancer immunology, immunotherapy, Vol.70(11), pp.3093-3103
- DOI
- 10.1007/s00262-021-02914-7
- PMID
- 33765210
- PMCID
- PMC8720267
- ISSN
- 0340-7004
- eISSN
- 1432-0851
- Grant note
- I01BX001702 / U.S. Department of Veterans Affairs I01 BX001702 / BLRD VA REU grant 1559927 / National Science Foundation P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 11/2021
- Academic Unit
- Microbiology and Immunology; President; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984297434902771
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