Activation of Gq/11 in the mouse corpus luteum is required for parturition

Rachel Mejia; Courtney Waite; Mario Ascoli

doi:10.1210/me.2014-1324

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Activation of Gq/11 in the mouse corpus luteum is required for parturition

Journal article

Open access

Peer reviewed

Activation of Gq/11 in the mouse corpus luteum is required for parturition

Rachel Mejia, Courtney Waite and Mario Ascoli

Molecular endocrinology (Baltimore, Md.), Vol.29(2), pp.238-246

02/2015

DOI: 10.1210/me.2014-1324

PMCID: PMC4318882

PMID: 25495873

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Abstract

Mice with a deletion of Gα(q/11) in granulosa cells were previously shown to be subfertile. They also have a reduced ovulatory response due to a deficiency in the ability of the activated LH receptor to fully induce the granulosa cell progesterone receptor. Because this conditional deletion of Gα(q/11) will interfere with the actions of any G protein-coupled receptor that activates G(q/11) in granulosa or luteal cells, we sought to determine whether the actions of other hormones that contribute to fertility were also impaired. We focused our attention on prostaglandin F2 (PGF2)α, because this hormone is known to activate phospholipase C (a prominent Gα(q/11) effector) in luteal cells and because the action of PGF2α on luteal cells is the first step in the murine parturition pathway. Our data show that the conditional deletion of Gα(q/11) from granulosa cells prevents the ability of PGF2α to induce Akr1c18 in luteal cells. Akr1c18 codes for 20α-hydroxysteroid dehydrogenase, an enzyme that inactivates progesterone. The PGF2α-mediated induction of this enzyme towards the end of pregnancy increases the inactivation of progesterone and precipitates parturition in mice. Thus, the conditional deletion of Gαq/11 from granulosa/luteal cells prevents the progesterone withdrawal that occurs at the end of pregnancy and impairs parturition. This novel molecular defect contributes to the subfertile phenotype of the mice with a deletion of Gα(q/11) from granulosa cells.

Pregnancy

Biomarkers - metabolism

Corpus Luteum - drug effects

Humans

Receptors, LH - metabolism

Progesterone - blood

Dinoprost - metabolism

Prolactin - pharmacology

Granulosa Cells - drug effects

Granulosa Cells - metabolism

Chorionic Gonadotropin - pharmacology

Corpus Luteum - cytology

Animals

Corpus Luteum - metabolism

Models, Biological

Gene Deletion

Female

Integrases - metabolism

Mice

Parturition - drug effects

GTP-Binding Protein alpha Subunits, Gq-G11 - metabolism

Inositol Phosphates - metabolism

Details

Title: Subtitle: Activation of Gq/11 in the mouse corpus luteum is required for parturition
Creators: Rachel Mejia - University of Iowa, Carver College of Medicine
Courtney Waite
Mario Ascoli - University of Iowa, Carver College of Medicine
Resource Type: Journal article
Publication Details: Molecular endocrinology (Baltimore, Md.), Vol.29(2), pp.238-246
DOI: 10.1210/me.2014-1324
PMID: 25495873
PMCID: PMC4318882
NLM abbreviation: Mol Endocrinol
ISSN: 0888-8809
eISSN: 1944-9917
Publisher: United States
Grant note: K12 HD063117 / NICHD NIH HHS
Language: English
Date published: 02/2015
Academic Unit: Obstetrics and Gynecology; Neuroscience and Pharmacology
Record Identifier: 9983930397702771

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