Journal article
Activation of NADPH Oxidase 1 Increases Intracellular Calcium and Migration of Smooth Muscle Cells
Hypertension (Dallas, Tex. 1979), Vol.58(3), pp.446-453
08/01/2011
DOI: 10.1161/HYPERTENSIONAHA.111.177006
PMCID: PMC3160712
PMID: 21810651
Abstract
Redox-dependent migration and proliferation of vascular smooth muscle cells (SMCs) are central events in the development of vascular proliferative diseases; however, the underlying intracellular signaling mechanisms are not fully understood. We tested the hypothesis that activation of Nox1 NADPH oxidase modulates intracellular calcium levels ([Ca
2+
]
i
). Using cultured SMCs from wild type (WT) and Nox1 null (Nox1
−/y
) mice, we confirmed that thrombin-dependent generation of ROS requires Nox1. Thrombin rapidly increased [Ca
2+
]
i
, as measured by fura-2 fluorescence ratio imaging, in WT but not Nox1 null SMCs. The increase in [Ca
2+
]
i
in WT SMCs was inhibited by antisense to Nox1 and restored by expression of Nox1 in Nox1 null SMCs. Investigation into potential mechanisms by which Nox1 modulates [Ca
2+
]
i
showed that thrombin-induced inositol triphosphate generation and thapsigargin-induced intracellular calcium mobilization were similar in WT and Nox1 null SMCs. To examine the effects of Nox1 on Ca
2+
entry, cells were either bathed in Ca
2+
-free media or exposed to dihydropyridines to block L-type Ca
2+
channel activity. Treatment with nifedipine or removal of extracellular Ca
2+
reduced the thrombin-mediated increase of [Ca
2+
]
i
in WT SMCs, whereas the response in Nox1 null SMCs was unchanged. Sodium vanadate, an inhibitor of protein tyrosine phosphatases, restored the thrombin-induced increase of [Ca
2+
]
i
in Nox1 null SMCs. Migration of SMCs was impaired with deficiency of Nox1 and restored with expression of Nox1 or addition of sodium vanadate. In summary, we conclude that Nox1 NADPH oxidase modulates Ca
2+
mobilization in SMCs, in part through regulation of Ca
2+
influx, to thereby promote cell migration.
Details
- Title: Subtitle
- Activation of NADPH Oxidase 1 Increases Intracellular Calcium and Migration of Smooth Muscle Cells
- Creators
- Matthew C. Zimmerman - University of IowaMaysam Takapoo - Iowa City VA Health Care SystemDammanahalli K. Jagadeesha - Department of Anatomy and Cell BiologyBojana Stanic - University of IowaBotond Banfi - University of IowaRamesh C. Bhalla - University of IowaFrancis J. Miller - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Hypertension (Dallas, Tex. 1979), Vol.58(3), pp.446-453
- DOI
- 10.1161/HYPERTENSIONAHA.111.177006
- PMID
- 21810651
- PMCID
- PMC3160712
- ISSN
- 0194-911X
- eISSN
- 1524-4563
- Grant note
- R01 HL081750-05 || HL / National Heart, Lung, and Blood Institute : NHLBI R01 HL081750-04 || HL / National Heart, Lung, and Blood Institute : NHLBI
- Language
- English
- Date published
- 08/01/2011
- Academic Unit
- Anatomy and Cell Biology; Otolaryngology; Internal Medicine
- Record Identifier
- 9984284346502771
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