Activation of alveolar macrophages and lung host defenses using transfer of the interferon-gamma gene

Dinghua Lei; Jack R Lancaster Jr; Mahesh S Joshi; Steve Nelson; David Stoltz; Gregory J Bagby; Guy Odom; Judd E Shellito; Jay K Kolls

doi:10.1152/ajplung.1997.272.5.l852

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Activation of alveolar macrophages and lung host defenses using transfer of the interferon-gamma gene

Journal article

Peer reviewed

Activation of alveolar macrophages and lung host defenses using transfer of the interferon-gamma gene

Dinghua Lei, Jack R Lancaster Jr, Mahesh S Joshi, Steve Nelson, David Stoltz, Gregory J Bagby, Guy Odom, Judd E Shellito and Jay K Kolls

The American journal of physiology, Vol.272(5 Pt 1), pp.L852-L859

05/1997

DOI: 10.1152/ajplung.1997.272.5.l852

PMID: 9176248

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Abstract

Interferon-gamma (IFN-gamma) is a critical cytokine in pulmonary host defenses against both intracellular and extracellular pathogens. To investigate whether this cytokine could be used therapeutically, we constructed an E1-deleted recombinant adenovirus encoding murine IFN-gamma. After intratracheal inoculation in rats, this vector resulted in prolonged expression of functional cytokine in vivo, as demonstrated by increased alveolar macrophage class II major histocompatibility complex expression, enhanced release of tumor necrosis factor in response to lipopolysaccharide, and enhanced host defenses against Pseudomonas aeruginosa. We postulate that this vector may be useful to study the role of exogenous IFN-gamma in a variety of pulmonary intracellular and extracellular pathogens.

Animals

Endotoxins - pharmacology

Gene Transfer Techniques

Immune System - physiology

Interferon-gamma - genetics

Interferon-gamma - pharmacokinetics

Interferon-gamma - physiology

Lung - immunology

Macrophages, Alveolar - physiology

Male

Pneumonia - etiology

Pseudomonas aeruginosa - immunology

Rats

Rats, Sprague-Dawley

Tumor Necrosis Factor-alpha - metabolism

Details

Title: Subtitle: Activation of alveolar macrophages and lung host defenses using transfer of the interferon-gamma gene
Creators: Dinghua Lei - Section of Pulmonary/Critical Care, Louisiana State University, New Orleans 70112, USA
Jack R Lancaster Jr - Louisiana State University Health Sciences Center New Orleans
Mahesh S Joshi - Louisiana State University Health Sciences Center New Orleans
Steve Nelson - Louisiana State University Health Sciences Center New Orleans
David Stoltz - Louisiana State University
Gregory J Bagby - Louisiana State University Health Sciences Center New Orleans
Guy Odom - Louisiana State University Health Sciences Center New Orleans
Judd E Shellito - Louisiana State University Health Sciences Center New Orleans
Jay K Kolls - Louisiana State University Health Sciences Center New Orleans
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.272(5 Pt 1), pp.L852-L859
DOI: 10.1152/ajplung.1997.272.5.l852
PMID: 9176248
ISSN: 0002-9513
eISSN: 2163-5773
Grant note: R29-AA10384 / NIAAA NIH HHS DK-46935-03 / NIDDK NIH HHS HL-29246 / NHLBI NIH HHS
Language: English
Date published: 05/1997
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
Record Identifier: 9984297503702771

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