Active sites of the cyclic GMP phosphodiesterase γ-subunit of retinal rod outer segments

V.M Lipkin; I.L Dumler; K.G Muradov; N.O Artemyev; R.N Etingof

doi:10.1016/0014-5793(88)80100-5

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Active sites of the cyclic GMP phosphodiesterase γ-subunit of retinal rod outer segments

Journal article

Open access

Peer reviewed

Active sites of the cyclic GMP phosphodiesterase γ-subunit of retinal rod outer segments

V.M Lipkin, I.L Dumler, K.G Muradov, N.O Artemyev and R.N Etingof

FEBS letters, Vol.234(2), pp.287-290

1988

DOI: 10.1016/0014-5793(88)80100-5

PMID: 2455657

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Abstract

Monoclonal antibodies were prepared to the γ-subunit of the cGMP phosphodiesterase. One of them γp-1, suppresses the activation of phosphodiesterase through the α-subunit of transducin. The γ-subunit fragment 24–45 rich in Arg and Lys residues is involved in γp-1 binding and is essential for the γ-subunit interaction with transducin. Carboxypeptidase Y cleaves off seven amino acid residues from the C-terminus of the γ-subunit resulting in phosphodiesterase activation. Thus, the C-terminal fragment of γ-subunit participates in phosphodiesterase inhibition.

Transducin

Cyclic-GMP phosphodiesterase

Monoclonal antibody

Carboxypeptidase Y

Details

Title: Subtitle: Active sites of the cyclic GMP phosphodiesterase γ-subunit of retinal rod outer segments
Creators: V.M Lipkin - Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, ul. Miklukho-Maklaya, 16/10, 117871 GSP Moscow V-437 USSR
I.L Dumler - Sechenov Institute of Evolutionary Physiology and Biochemistry, USSR Academy of Sciences, prosp. Moresa Toreza, 44, 194223 Leningrad, USSR
K.G Muradov - Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, ul. Miklukho-Maklaya, 16/10, 117871 GSP Moscow V-437 USSR
N.O Artemyev - Sechenov Institute of Evolutionary Physiology and Biochemistry, USSR Academy of Sciences, prosp. Moresa Toreza, 44, 194223 Leningrad, USSR
R.N Etingof - Sechenov Institute of Evolutionary Physiology and Biochemistry, USSR Academy of Sciences, prosp. Moresa Toreza, 44, 194223 Leningrad, USSR
Resource Type: Journal article
Publication Details: FEBS letters, Vol.234(2), pp.287-290
Publisher: Elsevier B.V
DOI: 10.1016/0014-5793(88)80100-5
PMID: 2455657
ISSN: 0014-5793
eISSN: 1873-3468
Language: English
Date published: 1988
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984025412102771

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